Effects of surface roughening and calcite coating of titanium on cell growth and differentiation

Rui Shi, Koichiro Hayashi, L. T. Bang, Kunio Ishikawa

研究成果: ジャーナルへの寄稿記事

抄録

Medical pure titanium (Ti) exhibits excellent mechanical properties and chemical stability in clinical use, but its initial osteointegration period is often postponed due to the bioinert nature of the Ti surface. Roughening and bioactive material coating of Ti implant surfaces are considered effective to enhance the bioactivity of Ti implants. In this study, we evaluated the effects of surface roughening and calcite (CaCO3) coating of Ti substrates on osteoblastic cell differentiation and growth. We roughened the Ti substrate surface by acid etching, followed by coating with calcite by thermal decomposition of Ca(NO3)2 to CaO followed by thermal carbonation of CaO to CaCO3. The surface topography of roughened Ti substrates (rough Ti) fluctuated, and the arithmetic average of surface roughness (Ra) was 2.2 µm. The rough topography was retained even after calcite coating of rough Ti, and the calcite-coated Ti (calcite-Ti) has an Ra of 2.0 µm. The tensile adhesive and shear adhesive strengths between calcite coating and Ti surface in calcite–Ti were 56.6 ± 16.1 and 10.1 ± 1.39 MPa, respectively. The biological properties and response of calcite–Ti were evaluated in vitro using a pre-osteoblastic cell line (MC3T3-E1). Observation of cell morphology by scanning electron microscopy and immunofluorescence staining revealed that MC3T3-E1 cells attached favorably to the surface with polygonal and filopodial extensions on calcite–Ti. The combination of roughening and calcite coating of the Ti substrate surface significantly increased cell proliferation at 1, 3, and 7 days of incubation. Furthermore, the relative alkaline phosphatase activity of calcite–Ti was higher than that of untreated Ti substrates (smooth Ti) and rough Ti after incubation for 7 days. Thus, the combined surface roughening and calcite coating of Ti substrates promoted MC3T3-E1 differentiation, whereas roughening alone was not effective.

元の言語英語
ジャーナルJournal of Biomaterials Applications
DOI
出版物ステータス受理済み/印刷中 - 1 1 2019

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Calcium Carbonate
Calcite
Cell growth
Titanium
Coatings
Substrates
Adhesives
Carbonation
Chemical stability
Phosphatases
Cell proliferation
Surface topography
Bioactivity
Topography
Alkaline Phosphatase
Etching
Pyrolysis
Surface roughness

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

これを引用

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abstract = "Medical pure titanium (Ti) exhibits excellent mechanical properties and chemical stability in clinical use, but its initial osteointegration period is often postponed due to the bioinert nature of the Ti surface. Roughening and bioactive material coating of Ti implant surfaces are considered effective to enhance the bioactivity of Ti implants. In this study, we evaluated the effects of surface roughening and calcite (CaCO3) coating of Ti substrates on osteoblastic cell differentiation and growth. We roughened the Ti substrate surface by acid etching, followed by coating with calcite by thermal decomposition of Ca(NO3)2 to CaO followed by thermal carbonation of CaO to CaCO3. The surface topography of roughened Ti substrates (rough Ti) fluctuated, and the arithmetic average of surface roughness (Ra) was 2.2 µm. The rough topography was retained even after calcite coating of rough Ti, and the calcite-coated Ti (calcite-Ti) has an Ra of 2.0 µm. The tensile adhesive and shear adhesive strengths between calcite coating and Ti surface in calcite–Ti were 56.6 ± 16.1 and 10.1 ± 1.39 MPa, respectively. The biological properties and response of calcite–Ti were evaluated in vitro using a pre-osteoblastic cell line (MC3T3-E1). Observation of cell morphology by scanning electron microscopy and immunofluorescence staining revealed that MC3T3-E1 cells attached favorably to the surface with polygonal and filopodial extensions on calcite–Ti. The combination of roughening and calcite coating of the Ti substrate surface significantly increased cell proliferation at 1, 3, and 7 days of incubation. Furthermore, the relative alkaline phosphatase activity of calcite–Ti was higher than that of untreated Ti substrates (smooth Ti) and rough Ti after incubation for 7 days. Thus, the combined surface roughening and calcite coating of Ti substrates promoted MC3T3-E1 differentiation, whereas roughening alone was not effective.",
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