Effects of symbiosis with Rhizobium fredii on transport of fixed nitrogen in the xylem of soybean plants

Takeo Yamakawa, Teiko Shirai, Junji Ishizuka

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

An experiment was conducted to identify the main nitrogenous compound transported in the xylem sap of soybean plants nodulated with Rhizobium fredii. Soybean (Glycine max L. Merr.) cultivars, wild type Bragg (nod+, fix+) and its nitrate tolerant, hypernodulating mutant nts1116 (nod++, fix+) were used for this experiment. These soybean plants were inoculated with a slow-growing rhizobium, Bradyrhizobium japonicum USDA110 or fast-growing rhizobia consisting of a mixture of R. fredii USDA191, USDA193, and USDA194 and grown in a phytotron under natural light and controlled temperature conditions. Xylem sap was collected from Bragg and nts1116 plants at the flowering and pod elongation stages. Acetylene reduction activity per plant or per nodule weight was not different between soybean lines and inoculums. The composition of the nitrogenous compounds in the xylem sap was compared between the symbionts, with B. japonicum and R. fredii. At the flowering stage, ureide-N and amide-N accounted for 53 to 70% and 20 to 27% respectively of the total N in the sap collected from the plants inoculated either with B. japonicum or R. fredii. At the pod elongation stage, ureide-N and amide-N accounted for 74 to 85%, and 7 to 19% of total sap N. With the growth of the soybean plants, the ratio of ureide-N in the xylem sap increased. These results suggest that in the case of wild soybean and the hypernodulating mutant line nodulated by R. fredii, ureide is transported as the main nitrogenous compound of fixed nitrogen in the xylem sap in the same way as in plants nodulated with B. japonicum.

本文言語英語
ページ(範囲)885-892
ページ数8
ジャーナルSoil Science and Plant Nutrition
46
4
DOI
出版ステータス出版済み - 2000

All Science Journal Classification (ASJC) codes

  • 土壌科学
  • 植物科学

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