Effects of temperature and nutrient concentration on the structural characteristics and removal of vegetable-associated Pseudomonas biofilm

Daisuke Hamanaka, Mami Onishi, Takuma Genkawa, Fumihiko Tanaka, Toshitaka Uchino

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

The development of vegetable-associated Pseudomonas biofilms on polystyrene surfaces with different nutrient concentrations (trypticase soy broth; TSB, and 1:20 diluted TSB) was evaluated at 5 and 30 °C for up to 5 days. Biofilm structural characteristics were observed by confocal scanning laser microscopy (CSLM) before and after water-removal treatment. Before removal treatment, the bacterial counts of biofilm at 30 °C were 0.5-1 logs greater than that at 5 °C throughout incubation. However, the detected cell counts of biofilm developed with diluted TSB were greater than that with TSB at both 5 and 30 °C. Although the difference in the structure of developed biofilm between TSB and diluted TSB was observed at 30 °C, it was not clear that the removal treatment affected Pseudomonas biofilm structure.

元の言語英語
ページ(範囲)165-170
ページ数6
ジャーナルFood Control
24
発行部数1-2
DOI
出版物ステータス出版済み - 3 1 2012

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Biofilms
Pseudomonas
Vegetables
biofilm
nutrient content
vegetables
Food
Temperature
temperature
Bacterial Load
Water Purification
Polystyrenes
polystyrenes
confocal laser scanning microscopy
Confocal Microscopy
plate count
Cell Count
water
cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science

これを引用

Effects of temperature and nutrient concentration on the structural characteristics and removal of vegetable-associated Pseudomonas biofilm. / Hamanaka, Daisuke; Onishi, Mami; Genkawa, Takuma; Tanaka, Fumihiko; Uchino, Toshitaka.

:: Food Control, 巻 24, 番号 1-2, 01.03.2012, p. 165-170.

研究成果: ジャーナルへの寄稿記事

Hamanaka, Daisuke ; Onishi, Mami ; Genkawa, Takuma ; Tanaka, Fumihiko ; Uchino, Toshitaka. / Effects of temperature and nutrient concentration on the structural characteristics and removal of vegetable-associated Pseudomonas biofilm. :: Food Control. 2012 ; 巻 24, 番号 1-2. pp. 165-170.
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