TY - JOUR
T1 - Effects of tumor necrosis factor-α on cell proliferation, prostaglandins and matrix-metalloproteinases production in rat endometrial stromal cells cultured in vitro
AU - Gamo, Toru
AU - Yamauchi, Nobuhiko
AU - Nishimura, Kyohei
AU - Watanabe, Ryo
AU - Matsumoto, Kenji
AU - Oozono, Shinji
AU - Kubota, Kaiyu
AU - He, Pei Jian
AU - Soh, Tomoki
AU - Hattori, Masa Aki
PY - 2007/12/1
Y1 - 2007/12/1
N2 - Tumor necrosis factor-α (TNF-α) is known as a pluripotent cell mediator, and it is implicated in the control of uterine cell growth, differentiation and function during estrous cycle and pregnancy. In this study, we investigated the effect of TNF-α on endometrial stromal cells derived from rat uterus (rat endometrial stromal cells, RES). RES were isolated from rat endometrium at day 5 of pregnancy. Proliferation activities of RES were measured by using bromodeoxyuridine (BrdU) labeling kit, the productions of prostaglandin E2 (PGE2) and prostaglandin F2α (PGF 2α) were measured by enzyme immunoassay kits and the production of matrix metalloproteinases (MMPs) was analyzed by gelatin-zymography. TNF-α, as well as epidermal growth factor and fibroblast growth factor-2, significantly increased the proliferation activity of RES (P < 0.05). TNF-α selectively stimulated the production of PGE2 in RES (P < 0.05), but not the production of PGF2α. Additionally, TNF-α did not stimulate the production of MMPs in RES at the concentration of 5 ng/mL, compared with the control groups (P > 0.05). In conclusion, this study demonstrates several regulational functions of TNF-α on RES using in vitro culture system. The effects of TNF-α on proliferation and MMP production of RES have been shown for the first time. We believe that these results demonstrate part of the functions of TNF-α in endometrium and contribute to the better understanding of endometrial functions.
AB - Tumor necrosis factor-α (TNF-α) is known as a pluripotent cell mediator, and it is implicated in the control of uterine cell growth, differentiation and function during estrous cycle and pregnancy. In this study, we investigated the effect of TNF-α on endometrial stromal cells derived from rat uterus (rat endometrial stromal cells, RES). RES were isolated from rat endometrium at day 5 of pregnancy. Proliferation activities of RES were measured by using bromodeoxyuridine (BrdU) labeling kit, the productions of prostaglandin E2 (PGE2) and prostaglandin F2α (PGF 2α) were measured by enzyme immunoassay kits and the production of matrix metalloproteinases (MMPs) was analyzed by gelatin-zymography. TNF-α, as well as epidermal growth factor and fibroblast growth factor-2, significantly increased the proliferation activity of RES (P < 0.05). TNF-α selectively stimulated the production of PGE2 in RES (P < 0.05), but not the production of PGF2α. Additionally, TNF-α did not stimulate the production of MMPs in RES at the concentration of 5 ng/mL, compared with the control groups (P > 0.05). In conclusion, this study demonstrates several regulational functions of TNF-α on RES using in vitro culture system. The effects of TNF-α on proliferation and MMP production of RES have been shown for the first time. We believe that these results demonstrate part of the functions of TNF-α in endometrium and contribute to the better understanding of endometrial functions.
UR - http://www.scopus.com/inward/record.url?scp=36749063008&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36749063008&partnerID=8YFLogxK
U2 - 10.1002/jez.a.423
DO - 10.1002/jez.a.423
M3 - Article
C2 - 17963241
AN - SCOPUS:36749063008
VL - 307
SP - 699
EP - 707
JO - Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
JF - Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
SN - 1932-5223
IS - 12
ER -