Efficacy of recombinant bacille Calmette-Guérin vaccine secreting interleukin-15/antigen 85B fusion protein in providing protection against Mycobacterium tuberculosis

Ce Tang, Hisakata Yamada, Kensuke Shibata, Naoyoshi Maeda, Shinichi Yoshida, Worawidh Wajjwalku, Naoya Ohara, Takeshi Yamada, Taroh Kinoshita, Yasunobu Yoshikai

研究成果: ジャーナルへの寄稿記事

36 引用 (Scopus)

抄録

Protection against Mycobacterium tuberculosis not only depends on CD4 + T helper type 1 (Th1) cells but, also, on CD8+ T cells. Interleukin (IL)-15 has an important function in the maintenance of memory CD8+ T cells. In the present study, we examined the efficacy of recombinant Mycobacterium bovis bacille Calmette-Guérin (rBCG) secreting fusion protein antigen (Ag) 85B murine IL-15 (rBCG-Ag85B-IL15) in providing protection against M. tuberculosis infection. The levels of major histocompatibility (MHC) class Ib (H2-M3)-binding TB2- or MHC class Ia (H-2Db)- binding MPT64-specific CD8+ T cells producing interferon (IFN)-γ were significantly higher after immunization with rBCG-Ag85B-IL15 than after immunization with rBCG secreting Ag85B (rBCG-Ag85B). The levels of purified protein derivative- or Ag85B-specific CD4+ T cells producing IFN-γ were also higher in mice immunized with rBCG-Ag85B-IL15 than in mice immunized with rBCG-Ag85B. Mice immunized with rBCG-Ag85B-IL15 exhibited CD8+ and CD4+ T cells responses that were stronger than those in mice immunized with rBCG-Ag85B, as well as robust protection in the lung against intratracheal challenge of M. tuberculosis. Thus, rBCG-Ag85B-IL15 vaccination capable of inducing efficient cell-mediated immunity might be used as an effective vaccine for tuberculosis.

元の言語英語
ページ(範囲)1263-1274
ページ数12
ジャーナルJournal of Infectious Diseases
197
発行部数9
DOI
出版物ステータス出版済み - 5 1 2008

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BCG Vaccine
Interleukin-15
Mycobacterium bovis
Mycobacterium tuberculosis
Antigens
Proteins
T-Lymphocytes
Histocompatibility
Interferons
Immunization
Tuberculosis Vaccines
Th1 Cells
Mycobacterium Infections
Cellular Immunity
Vaccination
Maintenance

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

これを引用

Efficacy of recombinant bacille Calmette-Guérin vaccine secreting interleukin-15/antigen 85B fusion protein in providing protection against Mycobacterium tuberculosis. / Tang, Ce; Yamada, Hisakata; Shibata, Kensuke; Maeda, Naoyoshi; Yoshida, Shinichi; Wajjwalku, Worawidh; Ohara, Naoya; Yamada, Takeshi; Kinoshita, Taroh; Yoshikai, Yasunobu.

:: Journal of Infectious Diseases, 巻 197, 番号 9, 01.05.2008, p. 1263-1274.

研究成果: ジャーナルへの寄稿記事

Tang, C, Yamada, H, Shibata, K, Maeda, N, Yoshida, S, Wajjwalku, W, Ohara, N, Yamada, T, Kinoshita, T & Yoshikai, Y 2008, 'Efficacy of recombinant bacille Calmette-Guérin vaccine secreting interleukin-15/antigen 85B fusion protein in providing protection against Mycobacterium tuberculosis', Journal of Infectious Diseases, 巻. 197, 番号 9, pp. 1263-1274. https://doi.org/10.1086/586902
Tang, Ce ; Yamada, Hisakata ; Shibata, Kensuke ; Maeda, Naoyoshi ; Yoshida, Shinichi ; Wajjwalku, Worawidh ; Ohara, Naoya ; Yamada, Takeshi ; Kinoshita, Taroh ; Yoshikai, Yasunobu. / Efficacy of recombinant bacille Calmette-Guérin vaccine secreting interleukin-15/antigen 85B fusion protein in providing protection against Mycobacterium tuberculosis. :: Journal of Infectious Diseases. 2008 ; 巻 197, 番号 9. pp. 1263-1274.
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abstract = "Protection against Mycobacterium tuberculosis not only depends on CD4 + T helper type 1 (Th1) cells but, also, on CD8+ T cells. Interleukin (IL)-15 has an important function in the maintenance of memory CD8+ T cells. In the present study, we examined the efficacy of recombinant Mycobacterium bovis bacille Calmette-Gu{\'e}rin (rBCG) secreting fusion protein antigen (Ag) 85B murine IL-15 (rBCG-Ag85B-IL15) in providing protection against M. tuberculosis infection. The levels of major histocompatibility (MHC) class Ib (H2-M3)-binding TB2- or MHC class Ia (H-2Db)- binding MPT64-specific CD8+ T cells producing interferon (IFN)-γ were significantly higher after immunization with rBCG-Ag85B-IL15 than after immunization with rBCG secreting Ag85B (rBCG-Ag85B). The levels of purified protein derivative- or Ag85B-specific CD4+ T cells producing IFN-γ were also higher in mice immunized with rBCG-Ag85B-IL15 than in mice immunized with rBCG-Ag85B. Mice immunized with rBCG-Ag85B-IL15 exhibited CD8+ and CD4+ T cells responses that were stronger than those in mice immunized with rBCG-Ag85B, as well as robust protection in the lung against intratracheal challenge of M. tuberculosis. Thus, rBCG-Ag85B-IL15 vaccination capable of inducing efficient cell-mediated immunity might be used as an effective vaccine for tuberculosis.",
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AU - Tang, Ce

AU - Yamada, Hisakata

AU - Shibata, Kensuke

AU - Maeda, Naoyoshi

AU - Yoshida, Shinichi

AU - Wajjwalku, Worawidh

AU - Ohara, Naoya

AU - Yamada, Takeshi

AU - Kinoshita, Taroh

AU - Yoshikai, Yasunobu

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AB - Protection against Mycobacterium tuberculosis not only depends on CD4 + T helper type 1 (Th1) cells but, also, on CD8+ T cells. Interleukin (IL)-15 has an important function in the maintenance of memory CD8+ T cells. In the present study, we examined the efficacy of recombinant Mycobacterium bovis bacille Calmette-Guérin (rBCG) secreting fusion protein antigen (Ag) 85B murine IL-15 (rBCG-Ag85B-IL15) in providing protection against M. tuberculosis infection. The levels of major histocompatibility (MHC) class Ib (H2-M3)-binding TB2- or MHC class Ia (H-2Db)- binding MPT64-specific CD8+ T cells producing interferon (IFN)-γ were significantly higher after immunization with rBCG-Ag85B-IL15 than after immunization with rBCG secreting Ag85B (rBCG-Ag85B). The levels of purified protein derivative- or Ag85B-specific CD4+ T cells producing IFN-γ were also higher in mice immunized with rBCG-Ag85B-IL15 than in mice immunized with rBCG-Ag85B. Mice immunized with rBCG-Ag85B-IL15 exhibited CD8+ and CD4+ T cells responses that were stronger than those in mice immunized with rBCG-Ag85B, as well as robust protection in the lung against intratracheal challenge of M. tuberculosis. Thus, rBCG-Ag85B-IL15 vaccination capable of inducing efficient cell-mediated immunity might be used as an effective vaccine for tuberculosis.

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