抄録
Engineering of hybrid gene clusters between the toluene metabolic tod operon and the biphenyl metabolic bph operon greatly enhanced the rate of biodegradation of trichloroethylene. Escherichia coli cells carrying a hybrid gene cluster composed of todC1 (the gene encoding the large subunit of toluene terminal dioxygenase in Pseudomonas putida F1), bphA2 (the gene encoding the small subunit of biphenyl terminal dioxygenase in Pseudomonas pseudoalcaligenes KF707), bphA3 (the gene encoding ferredoxin in KF707), and bphA4 (the gene encoding ferredoxin reductase in KF707) degraded trichloroethylene much faster than E. coli cells carrying the original toluene dioxygenase genes (todC1C2BA) or the original biphenyl dioxygenase genes (bphA1A2A3A4).
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 2121-2123 |
| ページ数 | 3 |
| ジャーナル | Journal of bacteriology |
| 巻 | 176 |
| 発行部数 | 7 |
| DOI |
|
| 出版物ステータス | 出版済み - 1 1 1994 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Molecular Biology
これを引用
Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase. / Furukawa, K.; Hirose, J.; Hayashida, S.; Nakamura, Kazuhiko.
：: Journal of bacteriology, 巻 176, 番号 7, 01.01.1994, p. 2121-2123.研究成果: ジャーナルへの寄稿 › コメント/討論
}
TY - JOUR
T1 - Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase
AU - Furukawa, K.
AU - Hirose, J.
AU - Hayashida, S.
AU - Nakamura, Kazuhiko
PY - 1994/1/1
Y1 - 1994/1/1
N2 - Engineering of hybrid gene clusters between the toluene metabolic tod operon and the biphenyl metabolic bph operon greatly enhanced the rate of biodegradation of trichloroethylene. Escherichia coli cells carrying a hybrid gene cluster composed of todC1 (the gene encoding the large subunit of toluene terminal dioxygenase in Pseudomonas putida F1), bphA2 (the gene encoding the small subunit of biphenyl terminal dioxygenase in Pseudomonas pseudoalcaligenes KF707), bphA3 (the gene encoding ferredoxin in KF707), and bphA4 (the gene encoding ferredoxin reductase in KF707) degraded trichloroethylene much faster than E. coli cells carrying the original toluene dioxygenase genes (todC1C2BA) or the original biphenyl dioxygenase genes (bphA1A2A3A4).
AB - Engineering of hybrid gene clusters between the toluene metabolic tod operon and the biphenyl metabolic bph operon greatly enhanced the rate of biodegradation of trichloroethylene. Escherichia coli cells carrying a hybrid gene cluster composed of todC1 (the gene encoding the large subunit of toluene terminal dioxygenase in Pseudomonas putida F1), bphA2 (the gene encoding the small subunit of biphenyl terminal dioxygenase in Pseudomonas pseudoalcaligenes KF707), bphA3 (the gene encoding ferredoxin in KF707), and bphA4 (the gene encoding ferredoxin reductase in KF707) degraded trichloroethylene much faster than E. coli cells carrying the original toluene dioxygenase genes (todC1C2BA) or the original biphenyl dioxygenase genes (bphA1A2A3A4).
UR - http://www.scopus.com/inward/record.url?scp=0028269085&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028269085&partnerID=8YFLogxK
U2 - 10.1128/jb.176.7.2121-2123.1994
DO - 10.1128/jb.176.7.2121-2123.1994
M3 - Comment/debate
C2 - 8144482
AN - SCOPUS:0028269085
VL - 176
SP - 2121
EP - 2123
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 7
ER -