Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase

K. Furukawa, J. Hirose, S. Hayashida, Kazuhiko Nakamura

研究成果: ジャーナルへの寄稿コメント/討論

54 引用 (Scopus)

抄録

Engineering of hybrid gene clusters between the toluene metabolic tod operon and the biphenyl metabolic bph operon greatly enhanced the rate of biodegradation of trichloroethylene. Escherichia coli cells carrying a hybrid gene cluster composed of todC1 (the gene encoding the large subunit of toluene terminal dioxygenase in Pseudomonas putida F1), bphA2 (the gene encoding the small subunit of biphenyl terminal dioxygenase in Pseudomonas pseudoalcaligenes KF707), bphA3 (the gene encoding ferredoxin in KF707), and bphA4 (the gene encoding ferredoxin reductase in KF707) degraded trichloroethylene much faster than E. coli cells carrying the original toluene dioxygenase genes (todC1C2BA) or the original biphenyl dioxygenase genes (bphA1A2A3A4).

元の言語英語
ページ(範囲)2121-2123
ページ数3
ジャーナルJournal of bacteriology
176
発行部数7
DOI
出版物ステータス出版済み - 1 1 1994

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Trichloroethylene
Dioxygenases
Genes
Ferredoxins
Operon
Multigene Family
Pseudomonas pseudoalcaligenes
Escherichia coli
Pseudomonas putida
Toluene
Oxidoreductases
diphenyl

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

これを引用

Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase. / Furukawa, K.; Hirose, J.; Hayashida, S.; Nakamura, Kazuhiko.

:: Journal of bacteriology, 巻 176, 番号 7, 01.01.1994, p. 2121-2123.

研究成果: ジャーナルへの寄稿コメント/討論

Furukawa, K. ; Hirose, J. ; Hayashida, S. ; Nakamura, Kazuhiko. / Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase. :: Journal of bacteriology. 1994 ; 巻 176, 番号 7. pp. 2121-2123.
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N2 - Engineering of hybrid gene clusters between the toluene metabolic tod operon and the biphenyl metabolic bph operon greatly enhanced the rate of biodegradation of trichloroethylene. Escherichia coli cells carrying a hybrid gene cluster composed of todC1 (the gene encoding the large subunit of toluene terminal dioxygenase in Pseudomonas putida F1), bphA2 (the gene encoding the small subunit of biphenyl terminal dioxygenase in Pseudomonas pseudoalcaligenes KF707), bphA3 (the gene encoding ferredoxin in KF707), and bphA4 (the gene encoding ferredoxin reductase in KF707) degraded trichloroethylene much faster than E. coli cells carrying the original toluene dioxygenase genes (todC1C2BA) or the original biphenyl dioxygenase genes (bphA1A2A3A4).

AB - Engineering of hybrid gene clusters between the toluene metabolic tod operon and the biphenyl metabolic bph operon greatly enhanced the rate of biodegradation of trichloroethylene. Escherichia coli cells carrying a hybrid gene cluster composed of todC1 (the gene encoding the large subunit of toluene terminal dioxygenase in Pseudomonas putida F1), bphA2 (the gene encoding the small subunit of biphenyl terminal dioxygenase in Pseudomonas pseudoalcaligenes KF707), bphA3 (the gene encoding ferredoxin in KF707), and bphA4 (the gene encoding ferredoxin reductase in KF707) degraded trichloroethylene much faster than E. coli cells carrying the original toluene dioxygenase genes (todC1C2BA) or the original biphenyl dioxygenase genes (bphA1A2A3A4).

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