Efficient delivery of signal-responsive gene carriers for disease-specific gene expression via bubble liposomes and sonoporation

Akira Tsuchiya, Jeong Hun Kang, Takeshi Mori, Yuki Naritomi, Satoshi Kushio, Takuro Niidome, Katsuro Tachibana, Yoko Takahashi, Yoichi Negishi, Yusuke Oda, Ryo Suzuki, Kazuo Maruyama, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Sonoporation is a promising method to intracellularly deliver synthetic gene carriers that have lower endocytotic uptake than viral carriers. Here, we applied sonoporation to deliver genes via polyethylene glycol (PEG)-grafted polymeric carriers that specifically respond to hyperactivated protein kinase A (PKA). PEG-grafted polymeric carrier/DNA polyplexes were not efficiently delivered into cells via the endocytotic pathway because of the hydrophilic PEG layer surrounding the polyplexes. However, the delivery of polyplexes into cells was significantly increased by sonoporation. The delivered polyplexes exhibited PKA-responsive transgene expression in PKA-overexpressing cells, but not in cells with low PKA activation. These results show that the sonoporation-mediated delivery of PEG-modified PKA-responsive polyplexes is a promising approach for safely applying gene therapy to abnormal cells with hyperactivated PKA.

元の言語英語
ページ(範囲)60-64
ページ数5
ジャーナルColloids and Surfaces B: Biointerfaces
160
DOI
出版物ステータス出版済み - 12 1 2017

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Liposomes
gene expression
Cyclic AMP-Dependent Protein Kinases
Gene expression
genes
delivery
bubbles
Genes
proteins
Proteins
Gene Expression
Polyethylene glycols
glycols
polyethylenes
cells
gene therapy
Synthetic Genes
Gene therapy
Transgenes
Genetic Therapy

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

これを引用

Efficient delivery of signal-responsive gene carriers for disease-specific gene expression via bubble liposomes and sonoporation. / Tsuchiya, Akira; Kang, Jeong Hun; Mori, Takeshi; Naritomi, Yuki; Kushio, Satoshi; Niidome, Takuro; Tachibana, Katsuro; Takahashi, Yoko; Negishi, Yoichi; Oda, Yusuke; Suzuki, Ryo; Maruyama, Kazuo; Katayama, Yoshiki.

:: Colloids and Surfaces B: Biointerfaces, 巻 160, 01.12.2017, p. 60-64.

研究成果: ジャーナルへの寄稿記事

Tsuchiya, Akira ; Kang, Jeong Hun ; Mori, Takeshi ; Naritomi, Yuki ; Kushio, Satoshi ; Niidome, Takuro ; Tachibana, Katsuro ; Takahashi, Yoko ; Negishi, Yoichi ; Oda, Yusuke ; Suzuki, Ryo ; Maruyama, Kazuo ; Katayama, Yoshiki. / Efficient delivery of signal-responsive gene carriers for disease-specific gene expression via bubble liposomes and sonoporation. :: Colloids and Surfaces B: Biointerfaces. 2017 ; 巻 160. pp. 60-64.
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abstract = "Sonoporation is a promising method to intracellularly deliver synthetic gene carriers that have lower endocytotic uptake than viral carriers. Here, we applied sonoporation to deliver genes via polyethylene glycol (PEG)-grafted polymeric carriers that specifically respond to hyperactivated protein kinase A (PKA). PEG-grafted polymeric carrier/DNA polyplexes were not efficiently delivered into cells via the endocytotic pathway because of the hydrophilic PEG layer surrounding the polyplexes. However, the delivery of polyplexes into cells was significantly increased by sonoporation. The delivered polyplexes exhibited PKA-responsive transgene expression in PKA-overexpressing cells, but not in cells with low PKA activation. These results show that the sonoporation-mediated delivery of PEG-modified PKA-responsive polyplexes is a promising approach for safely applying gene therapy to abnormal cells with hyperactivated PKA.",
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AU - Kushio, Satoshi

AU - Niidome, Takuro

AU - Tachibana, Katsuro

AU - Takahashi, Yoko

AU - Negishi, Yoichi

AU - Oda, Yusuke

AU - Suzuki, Ryo

AU - Maruyama, Kazuo

AU - Katayama, Yoshiki

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