Isotactic and syndiotactic poly(methyl methacrylate)s (PMMAs) form double-stranded helical stereocomplexes with a respective molar stoichiometry of 1:2 based on van der Waals interactions in organic solvents. Bioactivities of stereocomplex surfaces are significant for determining the potential for biomedical applications. We prepared PMMA stereocomplex films by a layer-by-layer assembly method, and the films were coated with protein A. Antibodies were deposited on the films, and then the binding of antigen was analyzed by QCM, AFM, and ATR-IR measurements. Specific binding amounts of HSA on Anti-HSA-coated stereocomplex films were greater than other surfaces and activity of the immobilized protein A on stereocomplex films was maintained for 10 days. These observations indicate that the surfaces of stereocomplex films were superior to conventional films for analysis of protein-protein interactions.
|出版ステータス||出版済み - 12月 1 2005|
|イベント||54th SPSJ Symposium on Macromolecules - Yamagata, 日本|
継続期間: 9月 20 2005 → 9月 22 2005
|その他||54th SPSJ Symposium on Macromolecules|
|Period||9/20/05 → 9/22/05|
!!!All Science Journal Classification (ASJC) codes