Efficient extracellular expression of a foreign protein in smptomyos using secretory protease inhibitor (SSI) gene fusions

Seiichi Taguchit, Izumi Kumagai, Jiro Nakayama, Akinori Suzuki, Kin Ichiro Miura

研究成果: Contribution to journalArticle査読

40 被引用数 (Scopus)

抄録

Using the gene for a secreted protease inhibitor, Streptomyces subtilisin inhibitor (SSI), we have established a secretory expression system for the products of foreign genes in Streptomyces. A sex pheromone peptide, cADl, of Enterococcus fae- calis was expressed and secreted as a stable complete fusion protein with SSI in amounts comparable to the native SSI in S. lividans 66. Recombinant cADl, isolated from the fusion protein by chemical digestion with BrCN, has the same amino acid composition and sequence, retention time on reverse phase HPLC, molecular mass, and biological activity as authentic cADl. This system should be useful for the efficient expression of other foreign gene products, and as a model for heterologous gene expression in Streptomyces.

本文言語英語
ページ(範囲)1063-1066
ページ数4
ジャーナルNature Biotechnology
7
10
DOI
出版ステータス出版済み - 10 1989
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー
  • 分子医療
  • 生体医工学

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