Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection

Jae Man Lee, Masateru Takahashi, Hiroaki Mon, Katsumi Koga, Yutaka Kawaguchi, Takahiro Kusakabe

研究成果: ジャーナルへの寄稿記事

12 引用 (Scopus)

抄録

Sonoporation (ultrasound treatment) provides a new and attractive nonviral way of in vivo gene transfer. To access the applicability of this method to the silkworm, Bombyx mori, we have compared the efficiencies of gene transfer by means of lipofection (using an appropriate agent, PDD111), sonoporation (ditto, FluoroGene™), and lipofection followed by sonoporation. By these methods, a luciferase expression plasmid was found to be markedly transferred into the haemocoel of newly ecdysed fifth instar silkworm larvae, and also into other tissues although with lower rates compared with the haemocoel. In terms of luciferase activity, the efficiencies of transgene by lipofection plus sonoporation were approximately 6 (hemocytes), 20 (silk glands), 8 (mid-gut), 38 (fat body), 10 (Malpighian tubules), 33 (ovaries), and 16 (testes) times as high as those by lipofection or sonoporation alone. These results demonstrated that the present method is useful to introduce the exogenous DNA into insect organs in vivo.

元の言語英語
ページ(範囲)976-979
ページ数4
ジャーナルCell Biology International
29
発行部数11
DOI
出版物ステータス出版済み - 11 1 2005

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Bombyx
Luciferases
Malpighian Tubules
Genes
Hemocytes
Fat Body
Silk
Transgenes
Larva
Insects
Testis
Ovary
Plasmids
DNA

All Science Journal Classification (ASJC) codes

  • Cell Biology

これを引用

Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection. / Lee, Jae Man; Takahashi, Masateru; Mon, Hiroaki; Koga, Katsumi; Kawaguchi, Yutaka; Kusakabe, Takahiro.

:: Cell Biology International, 巻 29, 番号 11, 01.11.2005, p. 976-979.

研究成果: ジャーナルへの寄稿記事

Lee, Jae Man ; Takahashi, Masateru ; Mon, Hiroaki ; Koga, Katsumi ; Kawaguchi, Yutaka ; Kusakabe, Takahiro. / Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection. :: Cell Biology International. 2005 ; 巻 29, 番号 11. pp. 976-979.
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