Efficient protein expression in Bombyx mori larvae of the strain d17 highly sensitive to B. mori nucleopolyhedrovirus

Naoya Kawakami, Jae Man Lee, Hiroaki Mon, Yuji Kubo, Yutaka Banno, Yutaka Kawaguchi, Katsumi Maenaka, Enoch Y. Park, Katsumi Koga, Takahiro Kusakabe

研究成果: ジャーナルへの寄稿記事

18 引用 (Scopus)

抄録

The recombinant protein expression by Bombyx mori nucleopolyhedrovirus (BmNPV) infecting silkworm larvae or pupae may endow us with a potent system for the production of large eukaryotic proteins. However, the screening of silkworm strains ideally suited to this method has scarcely been conducted. In the present study, we injected recombinant BmNPV containing a reporter gene, luciferase or DsRed, into hemocoel of fifth instar larvae of selected 12 silkworm strains. Among them, the strain d17 is found to be the highest in reporter expression from the intrinsic polyhedrin promoter of Autographa californica NPV or the silkworm actin A3 promoter. These results suggest that the d17 strain is highly permissive for BmNPV replication and is the most likely candidate of a "factory" for large-scale expression using the BmNPV bacmid system.

元の言語英語
ページ(範囲)180-185
ページ数6
ジャーナルMolecular Biotechnology
40
発行部数2
DOI
出版物ステータス出版済み - 10 1 2008

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Nucleopolyhedrovirus
Bombyx
Larva
Proteins
Recombinant proteins
Luciferases
Recombinant Proteins
Industrial plants
Actins
Screening
Genes
Pupa
Reporter Genes
fluorescent protein 583

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

これを引用

Efficient protein expression in Bombyx mori larvae of the strain d17 highly sensitive to B. mori nucleopolyhedrovirus. / Kawakami, Naoya; Lee, Jae Man; Mon, Hiroaki; Kubo, Yuji; Banno, Yutaka; Kawaguchi, Yutaka; Maenaka, Katsumi; Park, Enoch Y.; Koga, Katsumi; Kusakabe, Takahiro.

:: Molecular Biotechnology, 巻 40, 番号 2, 01.10.2008, p. 180-185.

研究成果: ジャーナルへの寄稿記事

Kawakami, Naoya ; Lee, Jae Man ; Mon, Hiroaki ; Kubo, Yuji ; Banno, Yutaka ; Kawaguchi, Yutaka ; Maenaka, Katsumi ; Park, Enoch Y. ; Koga, Katsumi ; Kusakabe, Takahiro. / Efficient protein expression in Bombyx mori larvae of the strain d17 highly sensitive to B. mori nucleopolyhedrovirus. :: Molecular Biotechnology. 2008 ; 巻 40, 番号 2. pp. 180-185.
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abstract = "The recombinant protein expression by Bombyx mori nucleopolyhedrovirus (BmNPV) infecting silkworm larvae or pupae may endow us with a potent system for the production of large eukaryotic proteins. However, the screening of silkworm strains ideally suited to this method has scarcely been conducted. In the present study, we injected recombinant BmNPV containing a reporter gene, luciferase or DsRed, into hemocoel of fifth instar larvae of selected 12 silkworm strains. Among them, the strain d17 is found to be the highest in reporter expression from the intrinsic polyhedrin promoter of Autographa californica NPV or the silkworm actin A3 promoter. These results suggest that the d17 strain is highly permissive for BmNPV replication and is the most likely candidate of a {"}factory{"} for large-scale expression using the BmNPV bacmid system.",
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AU - Kubo, Yuji

AU - Banno, Yutaka

AU - Kawaguchi, Yutaka

AU - Maenaka, Katsumi

AU - Park, Enoch Y.

AU - Koga, Katsumi

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