Eicosapentaenoic acid modifies cytokine activity and inhibits cell proliferation in an oesophageal cancer cell line

Hisako Kubota, Hideo Matsumoto, Masaharu Higashida, Haruaki Murakami, Hiroshi Nakashima, Yasuo Oka, Hideo Okumura, Masahiro Yamamura, Masahumi Nakamura, Toshihiro Hirai

研究成果: ジャーナルへの寄稿学術誌査読

22 被引用数 (Scopus)

抄録

Background/Aim: The present study investigated the effect of eicosapentaenoic acid (EPA) on nuclear factor-kappa B (NF-κB) activation, inflammatory interleukin-6 (IL-6) production, and cell proliferation in a human oesophageal carcinoma cell line (TE-1). Materials and Methods: Lipopolysaccharide (LPS)-induced IL-6 production in TE-1 cells in the presence or absence of EPA was determined using enzyme-linked immunosorbent assay (ELISA). The proliferation of TE-1 cells was determined by the WST-1 assay. TE-1 cells were stained with Hoechst 33342 and propidium iodide to observe apoptosis. Immunohistochemical staining of NF-κB in TE-1 cells was performed. Results: LPS increased IL-6 production in TE-1 cells, and EPA treatment prevented this effect. EPA treatment inhibited NF-κB activation and induced apoptosis of TE-1 cells. Conclusion: EPA inhibits NF-κB activation and IL-6 production in oesophageal cancer cells, their inducing apoptosis. These effects of EPA may be of benefit in improving the outcome of cancer surgery.

本文言語英語
ページ(範囲)4319-4324
ページ数6
ジャーナルAnticancer research
33
10
出版ステータス出版済み - 10月 1 2013
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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