Electrical detection of norovirus capsid using dielectrophoretic impedance measurement method

Michihiko Nakano, Takafumi Hisajima, Lina Mao, Junya Suehiro

研究成果: 著書/レポートタイプへの貢献会議での発言

4 引用 (Scopus)

抄録

In this work, we demonstrated an electrical detection technique of norovirus capsid in liquid by using dielectrophoretic impedance measurement (DEPIM). DEPIM is a method composing of capturing target particles by dielectrophoresis (DEP) in the gap of microelectrodes and measuring the impedance change of the microelectrodes caused by the captured particles. DEPIM has been successfully applied to bacteria detection by the present authors. This is the first report to extend scope of DEPIM inspection from bacteria to norovirus. Recombinant norovirus capsid was employed as target particles in experiments due to safety concerns. First, we showed virus capturing by DEP using fluorescent labeled norovirus capsid. Then, DEPIM of the capsid was carried out. As the result, DEPIM detected 2.5 ng/ml of the norovirus capsid in 300 sec. This implies that DEPIM has comparable sensitivity but shorter detection time than immunochromatography.

元の言語英語
ホスト出版物のタイトルIEEE SENSORS 2012 - Proceedings
DOI
出版物ステータス出版済み - 2012
イベント11th IEEE SENSORS 2012 Conference - Taipei, 台湾省、中華民国
継続期間: 10 28 201210 31 2012

その他

その他11th IEEE SENSORS 2012 Conference
台湾省、中華民国
Taipei
期間10/28/1210/31/12

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Microelectrodes
Electrophoresis
Bacteria
Viruses
Inspection
Liquids
Experiments

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

これを引用

Electrical detection of norovirus capsid using dielectrophoretic impedance measurement method. / Nakano, Michihiko; Hisajima, Takafumi; Mao, Lina; Suehiro, Junya.

IEEE SENSORS 2012 - Proceedings. 2012. 6411163.

研究成果: 著書/レポートタイプへの貢献会議での発言

Nakano, M, Hisajima, T, Mao, L & Suehiro, J 2012, Electrical detection of norovirus capsid using dielectrophoretic impedance measurement method. : IEEE SENSORS 2012 - Proceedings., 6411163, 11th IEEE SENSORS 2012 Conference, Taipei, 台湾省、中華民国, 10/28/12. https://doi.org/10.1109/ICSENS.2012.6411163
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