Electro protoplast fusion between Solanum sisymbriifolium and other Solanum species

Naoki Oda, Shiro Isshiki, Takeshi Sadohara, Yukio Ozaki, Hiroshi Okubo

研究成果: Contribution to journalArticle査読

抄録

Symmetric or asymmetric protoplast fusions between Solanum sisymbriifolium and other Solanum species (S. integrifolium and S. toxicarium) were performed to obtain the perfectly soil-borne disease resistant rootstocks for eggplant cultivation. Somatic hybrid was not obtained from symmetric fusion in selective media, where one parent's protoplasts formed colonies and the other did not. Combining of iodoacetamide-induced inactivation of S. integrifolium and no cell division ability of S. sisymbriifolium on a selective medium in asymmetric fusion, the hybridity of two calli was elucidated by analyzing IDH, PGM and SKDH isozymes. Plant regeneration from the somatic hybrid calli was also successful, but the regenerated plants did not show the hybridity. The result may be caused by the elimination of S. integrifolium chromosomes during the process of shoot regeneration from calli.

本文言語英語
ページ(範囲)67-72
ページ数6
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
51
1
出版ステータス出版済み - 2 1 2006

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 農業および作物学

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