Electrochemical parameters, formal potentials (Eo′), electron-transfer rate constants (ko′), and diffusion coefficients (Dct) have been determined for myoglobin (Mb) embedded in the Langmuir-Blodgett (LB) and cast films of five synthetic lipids including two types of polymeric lipids bearing ammonium or poly(ethylene glycol) as a head group. The heterogeneous electron-transfer rate constants of MbFeIII/MbFeII in these films were ca. 102-103-fold larger than those for Mb in solution at an indium tin oxide electrode. Myoglobin-lipid LB films on basal-plane pyrolytic graphite (PG) electrodes gave 3-10-fold larger ko′ values compared with cast films of the same lipid. The Mb-lipid films showed gel to liquid-crystal phase transitions consistent with bilayer structures. The peak current of the square wave voltammograms for the cast Mb-lipid films showed breaks in the phase-transition temperature regions of the corresponding lipid films. Dependence of ko′ on lipid structure was not significant. A variety of synthetic lipids provided suitable microenvironments for the enhanced electron transfer of Mb. Immobilized Mb in the lipid films was stable for more than a month. The specific orientation of Mb in all Mb-lipid films was shown by linear dichroism. The orientation does not depend on the type of the lipids.
|ジャーナル||Journal of the Chemical Society, Faraday Transactions|
|出版物ステータス||出版済み - 12 1 1995|
All Science Journal Classification (ASJC) codes
- Physical and Theoretical Chemistry