Electrochemical sensing for the determination of activated cyclic AMP-dependent protein kinase

Y. Katayama, S. Sakakihara, M. Maeda

    研究成果: ジャーナルへの寄稿学術誌査読

    13 被引用数 (Scopus)

    抄録

    A novel electrochemical system has been developed for monitoring the cyclic AMP-dependent protein kinase (PKA) activity. In this method, PKA activity was monitored as the change in the redox current of a ferrocene-pendant PKA substrate peptide (Fe-LRRASLG) on a gold electrode, which had been modified with thioctic acid, using cyclic voltammetry. The phosphrylation of the ferrocene-pendant substrate with PKA changed the net charge from +1 to -1. This caused a decrease in the redox current of the ferrocene unit due to an electrostatic repulsion between the substrate and the anionic surface of the electrode. We expect that this method is potentially useful for monitoring the enzyme activity in medical or pharmacological fields.

    本文言語英語
    ページ(範囲)17-19
    ページ数3
    ジャーナルanalytical sciences
    17
    1
    DOI
    出版ステータス出版済み - 2001

    !!!All Science Journal Classification (ASJC) codes

    • 分析化学

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