Electrochemical sensing for the determination of activated cyclic AMP-dependent protein kinase

Y. Katayama, S. Sakakihara, M. Maeda

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

A novel electrochemical system has been developed for monitoring the cyclic AMP-dependent protein kinase (PKA) activity. In this method, PKA activity was monitored as the change in the redox current of a ferrocene-pendant PKA substrate peptide (Fe-LRRASLG) on a gold electrode, which had been modified with thioctic acid, using cyclic voltammetry. The phosphrylation of the ferrocene-pendant substrate with PKA changed the net charge from +1 to -1. This caused a decrease in the redox current of the ferrocene unit due to an electrostatic repulsion between the substrate and the anionic surface of the electrode. We expect that this method is potentially useful for monitoring the enzyme activity in medical or pharmacological fields.

元の言語英語
ページ(範囲)17-19
ページ数3
ジャーナルanalytical sciences
17
発行部数1
DOI
出版物ステータス出版済み - 1 1 2001

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Cyclic AMP-Dependent Protein Kinases
Protein Kinases
Substrates
Thioctic Acid
Electrodes
Monitoring
Enzyme activity
Gold
Cyclic voltammetry
Electrostatics
Peptides
ferrocene
Oxidation-Reduction

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

これを引用

Electrochemical sensing for the determination of activated cyclic AMP-dependent protein kinase. / Katayama, Y.; Sakakihara, S.; Maeda, M.

:: analytical sciences, 巻 17, 番号 1, 01.01.2001, p. 17-19.

研究成果: ジャーナルへの寄稿記事

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