Electrochemical sensing of oxygen metabolism for a three-dimensional cultured model with biomimetic vascular flow

Yuji Nashimoto, Rei Mukomoto, Takuto Imaizumi, Takato Terai, Shotaro Shishido, Kosuke Ino, Ryuji Yokokawa, Takashi Miura, Kunishige Onuma, Masahiro Inoue, Hitoshi Shiku

研究成果: ジャーナルへの寄稿学術誌査読

1 被引用数 (Scopus)

抄録

Microphysiological systems (MPSs) with three-dimensional (3D) cultured models have attracted considerable interest because of their potential to mimic human health and disease conditions. Recent MPSs have shown significant advancements in engineering perfusable vascular networks integrated with 3D culture models, realizing a more physiological environment in vitro; however, a sensing system that can monitor their activity under biomimetic vascular flow is lacking. We designed an open-top microfluidic device with sensor capabilities and demonstrated its application in analyzing oxygen metabolism in vascularized 3D tissue models. We first validated the platform by using human lung fibroblast (hLF) spheroids. Then, we applied the platform to a patient-derived cancer organoid and evaluated the changes in oxygen metabolism during drug administration through the vascular network. We found that the platform could integrate a perfusable vascular network with 3D cultured cells, and the electrochemical sensor could detect the change in oxygen metabolism in a quantitative, non-invasive, and real-time manner. This platform would become a monitoring system for 3D cultured cells integrated with a perfusable vascular network.

本文言語英語
論文番号114808
ジャーナルBiosensors and Bioelectronics
219
DOI
出版ステータス出版済み - 1月 1 2023

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 生物理学
  • 生体医工学
  • 電気化学

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