Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water

Takeki Hamasaki, Gakuro Harada, Noboru Nakamichi, Shigeru Kabayama, Kiichiro Teruya, Bunshi Fugetsu, Wei Gong, Ichiro Sakata, Sanetaka Shirahata

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

Electrochemically reduced water (ERW) is produced near a cathode during electrolysis and exhibits an alkaline pH, contains richly dissolved hydrogen, and contains a small amount of platinum nanoparticles. ERW has reactive oxygen species (ROS)-scavenging activity and recent studies demonstrated that hydrogen-dissolved water exhibits ROS-scavenging activity. Thus, the antioxidative capacity of ERW is postulated to be dependent on the presence of hydrogen levels; however, there is no report verifying the role of dissolved hydrogen in ERW. In this report, we clarify whether the responsive factor for antioxidative activity in ERW is dissolved hydrogen. The intracellular ROS scavenging activity of ERW and hydrogen-dissolved water was tested by both fluorescent stain method and immuno spin trapping assay. We confirm that ERW possessed electrolysis intensity-dependent intracellular ROS-scavenging activity, and ERW exerts significantly superior ROS-scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water. ERW retained its ROS-scavenging activity after removal of dissolved hydrogen, but lost its activity when autoclaved. An oxygen radical absorbance capacity assay, the 2,2-diphenyl-1-picrylhydrazyl assay and chemiluminescence assay could not detect radical-scavenging activity in both ERW and hydrogen-dissolved water. These results indicate that ERW contains electrolysis-dependent hydrogen and an additional antioxidative factor predicted to be platinum nanoparticles.

元の言語英語
記事番号e0171192
ジャーナルPloS one
12
発行部数2
DOI
出版物ステータス出版済み - 2 1 2017

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Scavenging
hydrogen
Hydrogen
reactive oxygen species
Reactive Oxygen Species
Water
water
cells
Electrolysis
Assays
platinum
assays
nanoparticles
Platinum
Nanoparticles
Spin Trapping
oxygen radical absorbance capacity
Chemiluminescence
chemiluminescence
Luminescence

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water. / Hamasaki, Takeki; Harada, Gakuro; Nakamichi, Noboru; Kabayama, Shigeru; Teruya, Kiichiro; Fugetsu, Bunshi; Gong, Wei; Sakata, Ichiro; Shirahata, Sanetaka.

:: PloS one, 巻 12, 番号 2, e0171192, 01.02.2017.

研究成果: ジャーナルへの寄稿記事

Hamasaki, Takeki ; Harada, Gakuro ; Nakamichi, Noboru ; Kabayama, Shigeru ; Teruya, Kiichiro ; Fugetsu, Bunshi ; Gong, Wei ; Sakata, Ichiro ; Shirahata, Sanetaka. / Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water. :: PloS one. 2017 ; 巻 12, 番号 2.
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AU - Teruya, Kiichiro

AU - Fugetsu, Bunshi

AU - Gong, Wei

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