Electron microscopic single particle analysis of a tetrameric RuvA/RuvB/Holliday junction DNA complex

Kouta Mayanagi, Yoshie Fujiwara, Tomoko Miyata, Kosuke Morikawa

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

During the late stage of homologous recombination in prokaryotes, RuvA binds to the Holliday junction intermediate and executes branch migration in association with RuvB. The RuvA subunits form two distinct complexes with the Holliday junction: complex I with the single RuvA tetramer on one side of the four way junction DNA, and complex II with two tetramers on both sides. To investigate the functional roles of complexes I and II, we mutated two residues of RuvA (L125D and E126K) to prevent octamer formation. An electron microscopic analysis indicated that the mutant RuvA/RuvB/Holliday junction DNA complex formed the characteristic tripartite structure, with only one RuvA tetramer bound to one side of the Holliday junction, demonstrating the unexpected stability of this complex. The novel bent images of the complex revealed an intriguing morphological similarity to the structure of SV40 large T antigen, which belongs to the same AAA+ family as RuvB.

本文言語英語
ページ(範囲)273-278
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
365
2
DOI
出版ステータス出版済み - 1 11 2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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