TY - JOUR
T1 - Electron microscopic studies of giant nucleus-like structure formed by λ DNA introduced into the cytoplasma of Xenopus laevis fertilized eggs and embryos
AU - Shiokawa, Koichiro
AU - Tashiro, Kosuke
AU - Yamana, K.
AU - Sameshima, Munefumi
N1 - Funding Information:
The authors thank Dr. Yoshiyuki Sakaki, Research Laboratory for Genetic Information, School of Medicine, Kyushu University, Fukuoka, for warm encouragement throughout the experiments and for making facilities available for the present experiments. Thanks are also due to Miss Takayo Miura and Mr. Norihiko Nakakura for their help in some of the experiments. The present study was supported, in part, by a Grant-in-Aid for Special Project Research from the Ministry of Education, Science and Culture of Japan and a grant from Takeda Science Foundation (1986) to K.S. for the experiment of injecting exogenous DNA into Xenopus fertilized eggs.
PY - 1987/4
Y1 - 1987/4
N2 - When bacteriophage λ DNA was injected into the cytoplasm of the fertilized egg of Xenopus laevis, giant nucleus-like structures were assembled around the injected DNA. These nucleus-like structures survived during cleavage and were partitioned into blastomeres at the blastula stage. The nucleus-like structures formed in the uncleaved fertilized eggs and the blastula cells were both surrounded by a bilayer nuclear membrane with nuclear pore complexes. The ultrastructural features of the λ DNA-induced nucleus-like structure were considerably different from those of the normal blastula nucleus: although the nuclear pore complexes appeared to be normal, the 'nucleoplasm' was much too homogeneous as compared with that of the normal nucleus.
AB - When bacteriophage λ DNA was injected into the cytoplasm of the fertilized egg of Xenopus laevis, giant nucleus-like structures were assembled around the injected DNA. These nucleus-like structures survived during cleavage and were partitioned into blastomeres at the blastula stage. The nucleus-like structures formed in the uncleaved fertilized eggs and the blastula cells were both surrounded by a bilayer nuclear membrane with nuclear pore complexes. The ultrastructural features of the λ DNA-induced nucleus-like structure were considerably different from those of the normal blastula nucleus: although the nuclear pore complexes appeared to be normal, the 'nucleoplasm' was much too homogeneous as compared with that of the normal nucleus.
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U2 - 10.1016/0045-6039(87)90470-2
DO - 10.1016/0045-6039(87)90470-2
M3 - Article
C2 - 2953439
AN - SCOPUS:0023105491
VL - 20
SP - 253
EP - 261
JO - Cell Differentiation and Development
JF - Cell Differentiation and Development
SN - 0925-4773
IS - 4
ER -