Electrostatic adsorption of cytochrome c on ultrathin ZrO2-Gel layers and preparation of alternate multilayers

Izumi Ichinose, Rie Takaki, Keita Kuroiwa, Toyoki Kunitake

研究成果: Contribution to journalArticle査読

31 被引用数 (Scopus)


Positively charged proteins are electrostatically adsorbed on ultrathin metal oxide gel layers that are prepared by the surface sol-gel process. This feature was applied to assembly of multilayer films of cytochrome c (Cyt.c) and zirconium oxide. Regular growth of the alternate multilayer was verified by UV-vis absorption spectroscopy and quartz crystal microbalance measurements. The ZrO2gel layer was uniformly covered by the Cyt.c at pH 10.0 with a thickness of 2.4 nm for the protein layer. An excess amount of Cyt.c was adsorbed at pH 7.0 to produce globular aggregates on the ZrO2-gel layer, as confirmed by scanning electron microscopy. Cyt.c showed weak adsorption at pH 4.0, since the ZrO2gel layer had only a slight negative charge. When the ZrO2-gel layer was modified with octadecyltriethoxysilane, irreversible deposition of denatured Cyt.c was observed. In contrast, the ZrO2-gel covered by an ultrathin poly(vinyl alcohol) layer adsorbed Cyt.c without denaturation. Such Cyt.c molecules were readily desorbed by changing pH to above the isoelectric point. The surface sol-gel process provides a convenient method to immobilize water-soluble proteins through moderate electrostatic attraction.

出版ステータス出版済み - 4 29 2003

All Science Journal Classification (ASJC) codes

  • 材料科学(全般)
  • 凝縮系物理学
  • 表面および界面
  • 分光学
  • 電気化学


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