Elevation of intracellular calcium induced by the intrapipette perfusion technique modifies membrane ion currents in the chick cochlear hair cell

Takashi Kimitsuki, Takashi Nakagawa, Kazutaka Hisashi, Kohji Tsuji, Sizuo Komune, Sohtaro Komiyama

研究成果: Contribution to journalArticle

抜粋

The concentration of intracellular calcium ion ([Ca2+](i)) was altered in the same hair cell dissociated from a chick cochlea by the intrapipette perfusion technique. At a membrane potential of -40 mV, the elevation of [Ca2+](i) generated outward-going currents within 60 sec when the intrapipette solution was based on KCl. In controls, at membrane potentials more positive than -50 mV, outward K+ currents were observed and at large positive potentials, the outward K+ current decreased, showing an N-shaped I-V relationship. This outward K+ current was increased by elevation of [Ca2+](i) and was partially suppressed by a TEA-containing extracellular solution. We suggest that the Ca2+ increased by the intrapipette perfusion technique operates directly inside the cell membrane and activates Ca2+- activated K+ currents.

元の言語英語
ページ(範囲)70-73
ページ数4
ジャーナルActa Oto-Laryngologica
118
発行部数1
DOI
出版物ステータス出版済み - 3 18 1998

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

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