Elimination and utilization of oxidized guanine nucleotides in the synthesis of RNA and its precursors

Takeshi Sekiguchi, Riyoko Ito, Hiroshi Hayakawa, Mutsuo Sekiguchi

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Reactive oxygen species are produced as side products of oxygen utilization and can lead to the oxidation of nucleic acids and their precursor nucleotides. Among the various oxidized bases, 8-oxo-7,8-dihydroguanine seems to be the most critical during the transfer of genetic information because it can pair with both cytosine and adenine. During the de novo synthesis of guanine nucleotides, GMP is formed first, and it is converted to GDP by guanylate kinase. This enzyme hardly acts on an oxidized form ofGMP(8-oxo-GMP) formed by the oxidation ofGMPor by the cleavage of 8-oxo-GDP and 8-oxo-GTP by MutT protein. Although the formation of 8-oxo-GDP from 8-oxo-GMP is thus prevented, 8-oxo-GDP itself may be produced by the oxidation of GDP by reactive oxygen species. The 8-oxo-GDP thus formed can be converted to 8-oxo-GTP because nucleoside-diphosphate kinase and adenylate kinase, both of which catalyze the conversion of GDP to GTP, do not discriminate 8-oxo-GDP from normal GDP. The 8-oxo-GTP produced in this way and by the oxidation of GTP can be used for RNA synthesis. This misincorporation is prevented by MutT protein, which has the potential to cleave 8-oxo-GTP as well as 8-oxo-GDP to 8-oxo- GMP. When 14C-labeled 8-oxo-GTP was applied to CaCl2-permeabilized cells of a mutT- mutant strain, it could be incorporated into RNA at 4% of the rate for GTP. Escherichia coli cells appear to possess mechanisms to prevent misincorporation of 8-oxo-7,8-dihydroguanine into RNA.

元の言語英語
ページ(範囲)8128-8135
ページ数8
ジャーナルJournal of Biological Chemistry
288
発行部数12
DOI
出版物ステータス出版済み - 3 22 2013

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Guanine Nucleotides
RNA Precursors
Guanosine Triphosphate
Nucleoside-Diphosphate Kinase
Oxidation
RNA
Reactive Oxygen Species
Nucleic Acid Precursors
Guanylate Kinases
Adenylate Kinase
Cytosine
Adenine
Escherichia coli
Proteins
Nucleotides
Oxygen
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Elimination and utilization of oxidized guanine nucleotides in the synthesis of RNA and its precursors. / Sekiguchi, Takeshi; Ito, Riyoko; Hayakawa, Hiroshi; Sekiguchi, Mutsuo.

:: Journal of Biological Chemistry, 巻 288, 番号 12, 22.03.2013, p. 8128-8135.

研究成果: ジャーナルへの寄稿記事

Sekiguchi, Takeshi ; Ito, Riyoko ; Hayakawa, Hiroshi ; Sekiguchi, Mutsuo. / Elimination and utilization of oxidized guanine nucleotides in the synthesis of RNA and its precursors. :: Journal of Biological Chemistry. 2013 ; 巻 288, 番号 12. pp. 8128-8135.
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abstract = "Reactive oxygen species are produced as side products of oxygen utilization and can lead to the oxidation of nucleic acids and their precursor nucleotides. Among the various oxidized bases, 8-oxo-7,8-dihydroguanine seems to be the most critical during the transfer of genetic information because it can pair with both cytosine and adenine. During the de novo synthesis of guanine nucleotides, GMP is formed first, and it is converted to GDP by guanylate kinase. This enzyme hardly acts on an oxidized form ofGMP(8-oxo-GMP) formed by the oxidation ofGMPor by the cleavage of 8-oxo-GDP and 8-oxo-GTP by MutT protein. Although the formation of 8-oxo-GDP from 8-oxo-GMP is thus prevented, 8-oxo-GDP itself may be produced by the oxidation of GDP by reactive oxygen species. The 8-oxo-GDP thus formed can be converted to 8-oxo-GTP because nucleoside-diphosphate kinase and adenylate kinase, both of which catalyze the conversion of GDP to GTP, do not discriminate 8-oxo-GDP from normal GDP. The 8-oxo-GTP produced in this way and by the oxidation of GTP can be used for RNA synthesis. This misincorporation is prevented by MutT protein, which has the potential to cleave 8-oxo-GTP as well as 8-oxo-GDP to 8-oxo- GMP. When 14C-labeled 8-oxo-GTP was applied to CaCl2-permeabilized cells of a mutT- mutant strain, it could be incorporated into RNA at 4{\%} of the rate for GTP. Escherichia coli cells appear to possess mechanisms to prevent misincorporation of 8-oxo-7,8-dihydroguanine into RNA.",
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AB - Reactive oxygen species are produced as side products of oxygen utilization and can lead to the oxidation of nucleic acids and their precursor nucleotides. Among the various oxidized bases, 8-oxo-7,8-dihydroguanine seems to be the most critical during the transfer of genetic information because it can pair with both cytosine and adenine. During the de novo synthesis of guanine nucleotides, GMP is formed first, and it is converted to GDP by guanylate kinase. This enzyme hardly acts on an oxidized form ofGMP(8-oxo-GMP) formed by the oxidation ofGMPor by the cleavage of 8-oxo-GDP and 8-oxo-GTP by MutT protein. Although the formation of 8-oxo-GDP from 8-oxo-GMP is thus prevented, 8-oxo-GDP itself may be produced by the oxidation of GDP by reactive oxygen species. The 8-oxo-GDP thus formed can be converted to 8-oxo-GTP because nucleoside-diphosphate kinase and adenylate kinase, both of which catalyze the conversion of GDP to GTP, do not discriminate 8-oxo-GDP from normal GDP. The 8-oxo-GTP produced in this way and by the oxidation of GTP can be used for RNA synthesis. This misincorporation is prevented by MutT protein, which has the potential to cleave 8-oxo-GTP as well as 8-oxo-GDP to 8-oxo- GMP. When 14C-labeled 8-oxo-GTP was applied to CaCl2-permeabilized cells of a mutT- mutant strain, it could be incorporated into RNA at 4% of the rate for GTP. Escherichia coli cells appear to possess mechanisms to prevent misincorporation of 8-oxo-7,8-dihydroguanine into RNA.

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