ELISA, western blotting, immunocytolocalization and immunoaffinity column for naturally occuring bioactive compounds using monoclonal antibodies

Shao Jic Shan, Waraporn Putalun, Noriko Fukuda, Osamu Morinaga, Hiroyuki Tanaka, Yukihiro Shoyama

研究成果: ジャーナルへの寄稿評論記事

2 引用 (Scopus)

抄録

Anti-sennoside A (SA) and anti-glycyrrhizin (GC) MAbs were used for screening and cloning. And they were also used for ELISA. New western blotting method of determination for GC and ginsenoside was established that compounds separated by silica gel TLC were transferred to a polyvinylidene difluoride (PVDF) membrane. The membrane was treated with NaIO4 solution followed by BSA, resulting in a ginsenoside-BSA conjugate on the PVDF membrane which was stained by the general western blotting. Immunocytolocalization of GC in the fresh root of Glycyrrhiza species was conducted using anti-GC MAb after blotting to PVDF membrane. Immunoaffinity column chromatography using anti-ginsenoside Rb1 (GRb1)MAb performs better than previously published separation methods resulting in pure GRb1 from the crude extracts of ginseng. On the other hand, total solasodine glycosides were separated by an immunoaffinity column using a wide-cross reactive anti-solamargine (SM)MAb.

元の言語英語
ページ(範囲)258-269
ページ数12
ジャーナルJournal of Food and Drug Analysis
8
発行部数4
出版物ステータス出版済み - 12 1 2000

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glycyrrhizin
Glycyrrhizic Acid
monoclonal antibodies
Western blotting
Western Blotting
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
enzyme-linked immunosorbent assay
Ginsenosides
Membranes
Glycyrrhiza
Panax
Silica Gel
silica gel
Glycosides
Complex Mixtures
glycosides
Chromatography
Organism Cloning
molecular cloning

All Science Journal Classification (ASJC) codes

  • Food Science
  • Pharmacology

これを引用

ELISA, western blotting, immunocytolocalization and immunoaffinity column for naturally occuring bioactive compounds using monoclonal antibodies. / Shan, Shao Jic; Putalun, Waraporn; Fukuda, Noriko; Morinaga, Osamu; Tanaka, Hiroyuki; Shoyama, Yukihiro.

:: Journal of Food and Drug Analysis, 巻 8, 番号 4, 01.12.2000, p. 258-269.

研究成果: ジャーナルへの寄稿評論記事

Shan, Shao Jic ; Putalun, Waraporn ; Fukuda, Noriko ; Morinaga, Osamu ; Tanaka, Hiroyuki ; Shoyama, Yukihiro. / ELISA, western blotting, immunocytolocalization and immunoaffinity column for naturally occuring bioactive compounds using monoclonal antibodies. :: Journal of Food and Drug Analysis. 2000 ; 巻 8, 番号 4. pp. 258-269.
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abstract = "Anti-sennoside A (SA) and anti-glycyrrhizin (GC) MAbs were used for screening and cloning. And they were also used for ELISA. New western blotting method of determination for GC and ginsenoside was established that compounds separated by silica gel TLC were transferred to a polyvinylidene difluoride (PVDF) membrane. The membrane was treated with NaIO4 solution followed by BSA, resulting in a ginsenoside-BSA conjugate on the PVDF membrane which was stained by the general western blotting. Immunocytolocalization of GC in the fresh root of Glycyrrhiza species was conducted using anti-GC MAb after blotting to PVDF membrane. Immunoaffinity column chromatography using anti-ginsenoside Rb1 (GRb1)MAb performs better than previously published separation methods resulting in pure GRb1 from the crude extracts of ginseng. On the other hand, total solasodine glycosides were separated by an immunoaffinity column using a wide-cross reactive anti-solamargine (SM)MAb.",
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AU - Shan, Shao Jic

AU - Putalun, Waraporn

AU - Fukuda, Noriko

AU - Morinaga, Osamu

AU - Tanaka, Hiroyuki

AU - Shoyama, Yukihiro

PY - 2000/12/1

Y1 - 2000/12/1

N2 - Anti-sennoside A (SA) and anti-glycyrrhizin (GC) MAbs were used for screening and cloning. And they were also used for ELISA. New western blotting method of determination for GC and ginsenoside was established that compounds separated by silica gel TLC were transferred to a polyvinylidene difluoride (PVDF) membrane. The membrane was treated with NaIO4 solution followed by BSA, resulting in a ginsenoside-BSA conjugate on the PVDF membrane which was stained by the general western blotting. Immunocytolocalization of GC in the fresh root of Glycyrrhiza species was conducted using anti-GC MAb after blotting to PVDF membrane. Immunoaffinity column chromatography using anti-ginsenoside Rb1 (GRb1)MAb performs better than previously published separation methods resulting in pure GRb1 from the crude extracts of ginseng. On the other hand, total solasodine glycosides were separated by an immunoaffinity column using a wide-cross reactive anti-solamargine (SM)MAb.

AB - Anti-sennoside A (SA) and anti-glycyrrhizin (GC) MAbs were used for screening and cloning. And they were also used for ELISA. New western blotting method of determination for GC and ginsenoside was established that compounds separated by silica gel TLC were transferred to a polyvinylidene difluoride (PVDF) membrane. The membrane was treated with NaIO4 solution followed by BSA, resulting in a ginsenoside-BSA conjugate on the PVDF membrane which was stained by the general western blotting. Immunocytolocalization of GC in the fresh root of Glycyrrhiza species was conducted using anti-GC MAb after blotting to PVDF membrane. Immunoaffinity column chromatography using anti-ginsenoside Rb1 (GRb1)MAb performs better than previously published separation methods resulting in pure GRb1 from the crude extracts of ginseng. On the other hand, total solasodine glycosides were separated by an immunoaffinity column using a wide-cross reactive anti-solamargine (SM)MAb.

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