Embryos and embryonic stem cells from the white rhinoceros

Thomas B. Hildebrandt, Robert Hermes, Silvia Colleoni, Sebastian Diecke, Susanne Holtze, Marilyn B. Renfree, Jan Stejskal, Katsuhiko Hayashi, Micha Drukker, Pasqualino Loi, Frank Göritz, Giovanna Lazzari, Cesare Galli

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

The northern white rhinoceros (NWR, Ceratotherium simum cottoni) is the most endangered mammal in the world with only two females surviving. Here we adapt existing assisted reproduction techniques (ART) to fertilize Southern White Rhinoceros (SWR) oocytes with NWR spermatozoa. We show that rhinoceros oocytes can be repeatedly recovered from live SWR females by transrectal ovum pick-up, matured, fertilized by intracytoplasmic sperm injection and developed to the blastocyst stage in vitro. Next, we generate hybrid rhinoceros embryos in vitro using gametes of NWR and SWR. We also establish embryonic stem cell lines from the SWR blastocysts. Blastocysts are cryopreserved for later embryo transfer. Our results indicate that ART could be a viable strategy to rescue genes from the iconic, almost extinct, northern white rhinoceros and may also have broader impact if applied with similar success to other endangered large mammalian species.

元の言語英語
記事番号2589
ジャーナルNature communications
9
発行部数1
DOI
出版物ステータス出版済み - 12 1 2018

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gametocytes
Mammals
stem cells
embryos
Blastocyst
Embryonic Stem Cells
Stem cells
Reproductive Techniques
Embryonic Structures
Genes
spermatozoa
mammals
Oocytes
cultured cells
genes
Intracytoplasmic Sperm Injections
Embryo Transfer
injection
Germ Cells
Ovum

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

これを引用

Hildebrandt, T. B., Hermes, R., Colleoni, S., Diecke, S., Holtze, S., Renfree, M. B., ... Galli, C. (2018). Embryos and embryonic stem cells from the white rhinoceros. Nature communications, 9(1), [2589]. https://doi.org/10.1038/s41467-018-04959-2

Embryos and embryonic stem cells from the white rhinoceros. / Hildebrandt, Thomas B.; Hermes, Robert; Colleoni, Silvia; Diecke, Sebastian; Holtze, Susanne; Renfree, Marilyn B.; Stejskal, Jan; Hayashi, Katsuhiko; Drukker, Micha; Loi, Pasqualino; Göritz, Frank; Lazzari, Giovanna; Galli, Cesare.

:: Nature communications, 巻 9, 番号 1, 2589, 01.12.2018.

研究成果: ジャーナルへの寄稿記事

Hildebrandt, TB, Hermes, R, Colleoni, S, Diecke, S, Holtze, S, Renfree, MB, Stejskal, J, Hayashi, K, Drukker, M, Loi, P, Göritz, F, Lazzari, G & Galli, C 2018, 'Embryos and embryonic stem cells from the white rhinoceros', Nature communications, 巻. 9, 番号 1, 2589. https://doi.org/10.1038/s41467-018-04959-2
Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB その他. Embryos and embryonic stem cells from the white rhinoceros. Nature communications. 2018 12 1;9(1). 2589. https://doi.org/10.1038/s41467-018-04959-2
Hildebrandt, Thomas B. ; Hermes, Robert ; Colleoni, Silvia ; Diecke, Sebastian ; Holtze, Susanne ; Renfree, Marilyn B. ; Stejskal, Jan ; Hayashi, Katsuhiko ; Drukker, Micha ; Loi, Pasqualino ; Göritz, Frank ; Lazzari, Giovanna ; Galli, Cesare. / Embryos and embryonic stem cells from the white rhinoceros. :: Nature communications. 2018 ; 巻 9, 番号 1.
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