En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles

S. Hara-Kuge, O. Kuge, L. Orci, M. Amherdt, M. Ravazzola, F. T. Wieland, J. E. Rothman

研究成果: Contribution to journalArticle査読

105 被引用数 (Scopus)

抄録

The cDNA encoding ε-COP, the 36-kD subunit of coatomer, was cloned from a bovine liver cDNA library and sequenced. Immunoblotting with an anti-ε-COP antibody showed that ε-COP exists in COP-coated vesicles as well as in the cytosolic coatomer. Using the cloned cDNA, recombinant His6-tagged ε-COP was overexpressed in cultured Chinese hamster ovary (CHO) cells, from which metabolically radiolabeled coatomer was purified by taking advantage of the His6 tag. Radiolabeled coatomer was employed to establish that all the subunits of the coatomer enter coated vesicles as an intact unit.

本文言語英語
ページ(範囲)883-892
ページ数10
ジャーナルJournal of Cell Biology
124
6
DOI
出版ステータス出版済み - 1994
外部発表はい

All Science Journal Classification (ASJC) codes

  • Cell Biology

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