Endocrine therapy-resistant breast cancer model cells are inhibited by soybean glyceollin I through Eleanor non-coding RNA

Tatsuro Yamamoto, Chiyomi Sakamoto, Hiroaki Tachiwana, Mitsuru Kumabe, Toshiro Matsui, Tadatoshi Yamashita, Masatoshi Shinagawa, Koji Ochiai, Noriko Saitoh, Mitsuyoshi Nakao

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

Long-term estrogen deprivation (LTED) of an estrogen receptor (ER) α-positive breast cancer cell line recapitulates cancer cells that have acquired estrogen-independent cell proliferation and endocrine therapy resistance. Previously, we have shown that a cluster of non-coding RNAs, Eleanors (ESR1 locus enhancing and activating non-coding RNAs) formed RNA cloud and upregulated the ESR1 gene in the nuclei of LTED cells. Eleanors were inhibited by resveratrol through ER. Here we prepared another polyphenol, glyceollin I from stressed soybeans, and identified it as a major inhibitor of the Eleanor RNA cloud and ESR1 mRNA transcription. The inhibition was independent of ER, unlike one by resveratrol. This was consistent with a distinct tertiary structure of glyceollin I for ER binding. Glyceollin I preferentially inhibited the growth of LTED cells and induced apoptosis. Our results suggest that glyceollin I has a novel role in LTED cell inhibition through Eleanors. In other words, LTED cells or endocrine therapy-resistant breast cancer cells may be ready for apoptosis, which can be triggered with polyphenols both in ER-dependent and ER-independent manners.

元の言語英語
記事番号15202
ジャーナルScientific reports
8
発行部数1
DOI
出版物ステータス出版済み - 12 1 2018

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Untranslated RNA
Soybeans
Estrogen Receptors
Estrogens
Breast Neoplasms
Polyphenols
Therapeutics
RNA
Apoptosis
Endocrine Cells
glyceollin
Cell Proliferation
Cell Line
Messenger RNA
Growth
Genes
Neoplasms

All Science Journal Classification (ASJC) codes

  • General

これを引用

Endocrine therapy-resistant breast cancer model cells are inhibited by soybean glyceollin I through Eleanor non-coding RNA. / Yamamoto, Tatsuro; Sakamoto, Chiyomi; Tachiwana, Hiroaki; Kumabe, Mitsuru; Matsui, Toshiro; Yamashita, Tadatoshi; Shinagawa, Masatoshi; Ochiai, Koji; Saitoh, Noriko; Nakao, Mitsuyoshi.

:: Scientific reports, 巻 8, 番号 1, 15202, 01.12.2018.

研究成果: ジャーナルへの寄稿記事

Yamamoto, T, Sakamoto, C, Tachiwana, H, Kumabe, M, Matsui, T, Yamashita, T, Shinagawa, M, Ochiai, K, Saitoh, N & Nakao, M 2018, 'Endocrine therapy-resistant breast cancer model cells are inhibited by soybean glyceollin I through Eleanor non-coding RNA', Scientific reports, 巻. 8, 番号 1, 15202. https://doi.org/10.1038/s41598-018-33227-y
Yamamoto, Tatsuro ; Sakamoto, Chiyomi ; Tachiwana, Hiroaki ; Kumabe, Mitsuru ; Matsui, Toshiro ; Yamashita, Tadatoshi ; Shinagawa, Masatoshi ; Ochiai, Koji ; Saitoh, Noriko ; Nakao, Mitsuyoshi. / Endocrine therapy-resistant breast cancer model cells are inhibited by soybean glyceollin I through Eleanor non-coding RNA. :: Scientific reports. 2018 ; 巻 8, 番号 1.
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abstract = "Long-term estrogen deprivation (LTED) of an estrogen receptor (ER) α-positive breast cancer cell line recapitulates cancer cells that have acquired estrogen-independent cell proliferation and endocrine therapy resistance. Previously, we have shown that a cluster of non-coding RNAs, Eleanors (ESR1 locus enhancing and activating non-coding RNAs) formed RNA cloud and upregulated the ESR1 gene in the nuclei of LTED cells. Eleanors were inhibited by resveratrol through ER. Here we prepared another polyphenol, glyceollin I from stressed soybeans, and identified it as a major inhibitor of the Eleanor RNA cloud and ESR1 mRNA transcription. The inhibition was independent of ER, unlike one by resveratrol. This was consistent with a distinct tertiary structure of glyceollin I for ER binding. Glyceollin I preferentially inhibited the growth of LTED cells and induced apoptosis. Our results suggest that glyceollin I has a novel role in LTED cell inhibition through Eleanors. In other words, LTED cells or endocrine therapy-resistant breast cancer cells may be ready for apoptosis, which can be triggered with polyphenols both in ER-dependent and ER-independent manners.",
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