Endoglycoceramidase treatment inhibits synchronous oscillations of intracellular Ca2+ in cultured cortical neurons

Kazuyo Muramoto, Masahiro Kawahara, Kazuo Kobayashi, Makoto Ito, Tatsuya Yamagata, Yoichiro Kuroda

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Gangliosides are major components of nerve cell membranes and are especially rich in synaptic areas. In order to evaluate the role of endogenous gangliosides in synapse formation, endoglycoceramidase (EGCase) was used to remove oligosaccharides of gangliosides from the cell surface. We have reported previously that synapse formation between cultured rat cerebral cortical neurons can be estimated by the synchronous oscillation of synaptic activity monitored by fura-2 calcium imaging. Continuous application of endoglycoceramidase (EGCase) together with its activator protein dose- dependently decreased the frequency of synchronous oscillations without any morphological changes in neurons and their neurites. The result suggests that oligosaccharides liberated from glycosphingolipids on cultured cortical cell surface with EGCase are important for synapse formation between cortical neurons.

元の言語英語
ページ(範囲)398-402
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
202
発行部数1
DOI
出版物ステータス出版済み - 1 1 1994

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endoglycoceramidase
Neurons
Gangliosides
Synapses
Oligosaccharides
Glycosphingolipids
Fura-2
Neurites
Cell membranes
Rats
Cultured Cells
Cell Membrane
Calcium
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Endoglycoceramidase treatment inhibits synchronous oscillations of intracellular Ca2+ in cultured cortical neurons. / Muramoto, Kazuyo; Kawahara, Masahiro; Kobayashi, Kazuo; Ito, Makoto; Yamagata, Tatsuya; Kuroda, Yoichiro.

:: Biochemical and Biophysical Research Communications, 巻 202, 番号 1, 01.01.1994, p. 398-402.

研究成果: ジャーナルへの寄稿記事

Muramoto, Kazuyo ; Kawahara, Masahiro ; Kobayashi, Kazuo ; Ito, Makoto ; Yamagata, Tatsuya ; Kuroda, Yoichiro. / Endoglycoceramidase treatment inhibits synchronous oscillations of intracellular Ca2+ in cultured cortical neurons. :: Biochemical and Biophysical Research Communications. 1994 ; 巻 202, 番号 1. pp. 398-402.
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