Endoglycoceramidase treatment inhibits synchronous oscillations of intracellular Ca2+ in cultured cortical neurons

Kazuyo Muramoto, Masahiro Kawahara, Kazuo Kobayashi, Makoto Ito, Tatsuya Yamagata, Yoichiro Kuroda

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)


Gangliosides are major components of nerve cell membranes and are especially rich in synaptic areas. In order to evaluate the role of endogenous gangliosides in synapse formation, endoglycoceramidase (EGCase) was used to remove oligosaccharides of gangliosides from the cell surface. We have reported previously that synapse formation between cultured rat cerebral cortical neurons can be estimated by the synchronous oscillation of synaptic activity monitored by fura-2 calcium imaging. Continuous application of endoglycoceramidase (EGCase) together with its activator protein dose- dependently decreased the frequency of synchronous oscillations without any morphological changes in neurons and their neurites. The result suggests that oligosaccharides liberated from glycosphingolipids on cultured cortical cell surface with EGCase are important for synapse formation between cortical neurons.

ジャーナルBiochemical and Biophysical Research Communications
出版ステータス出版済み - 1 1 1994

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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