TY - JOUR
T1 - Engineered Coatings for Titanium Implants to Present Ultralow Doses of BMP-7
AU - Al-Jarsha, Mohammed
AU - Moulisová, Vladimíra
AU - Leal-Egaña, Aldo
AU - Connell, Andrew
AU - Naudi, Kurt B.
AU - Ayoub, Ashraf F.
AU - Dalby, Matthew J.
AU - Salmerón-Sánchez, Manuel
N1 - Funding Information:
We thank Annie Zhe Cheng (School of Engineering, University of Glasgow) for preparing the titanium discs, and Frankie Vanterpool (Biomedical Engineering, University of Glasgow) and Ross Gurden (Centre for Cell Engineering, University of Glasgow) for developing, testing and introducing “R script” for focal adhesion analysis. This study was supported by the European Research Council (ERC 306990), the U.K. Regenerative Medicine Platform (MRC grant MR/L022710/ 1), and an EPSRC Programme Grant (EP/P001114/1).
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/5/14
Y1 - 2018/5/14
N2 - The ongoing research to improve the clinical outcome of titanium implants has resulted in the implemetation of multiple approches to deliver osteogenic growth factors accelerating and sustaining osseointegration. Here we show the presentation of human bone morphogenetic protein 7 (BMP-7) adsorbed to titanium discs coated with poly(ethyl acrylate) (PEA). We have previously shown that PEA promotes fibronectin organization into nanonetworks exposing integrin- and growth-factor-binding domains, allowing a synergistic interaction at the integrin/growth factor receptor level. Here, titanium discs were coated with PEA and fibronectin and then decorated with ng/mL doses of BMP-7. Human mesenchymal stem cells were used to investigate cellular responses on these functionalized microenvironments. Cell adhesion, proliferation, and mineralization, as well as osteogenic markers expression (osteopontin and osteocalcin) revealed the ability of the system to be more potent in osteodifferentiation of the mesenchymal cells than combinations of titanium and BMP-7 in absence of PEA coatings. This work represents a novel strategy to improve the biological activity of titanium implants with BMP-7.
AB - The ongoing research to improve the clinical outcome of titanium implants has resulted in the implemetation of multiple approches to deliver osteogenic growth factors accelerating and sustaining osseointegration. Here we show the presentation of human bone morphogenetic protein 7 (BMP-7) adsorbed to titanium discs coated with poly(ethyl acrylate) (PEA). We have previously shown that PEA promotes fibronectin organization into nanonetworks exposing integrin- and growth-factor-binding domains, allowing a synergistic interaction at the integrin/growth factor receptor level. Here, titanium discs were coated with PEA and fibronectin and then decorated with ng/mL doses of BMP-7. Human mesenchymal stem cells were used to investigate cellular responses on these functionalized microenvironments. Cell adhesion, proliferation, and mineralization, as well as osteogenic markers expression (osteopontin and osteocalcin) revealed the ability of the system to be more potent in osteodifferentiation of the mesenchymal cells than combinations of titanium and BMP-7 in absence of PEA coatings. This work represents a novel strategy to improve the biological activity of titanium implants with BMP-7.
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U2 - 10.1021/acsbiomaterials.7b01037
DO - 10.1021/acsbiomaterials.7b01037
M3 - Article
AN - SCOPUS:85046483988
SN - 2373-9878
VL - 4
SP - 1812
EP - 1819
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 5
ER -