Engineered synthetic pathway for isopropanol production in Escherichia coli

Taizo Hanai, S. Atsumi, J. C. Liao

研究成果: ジャーナルへの寄稿記事

186 引用 (Scopus)

抄録

A synthetic pathway was engineered in Escherichia coli to produce isopropanol by expressing various combinations of genes from Clostridium acetobutyUcum ATCC 824, E. coli K-12 MG1655, Clostridium beijerinckii NRRL B593, and Thermoanaerobacter brockii HTD4. The strain with the combination of C. acetobutylicum thl (acetyl-coenzyme A [CoA] acetyltransferase), E. coli atoAD (acetoacetyl-CoA transferase), C. acetobutylicum adc (acetoacetate decarboxylase), and C. beijerinckii adh (secondary alcohol dehydrogenase) achieved the highest titer. This strain produced 81.6 mM isopropanol in shake flasks with a yield of 43.5% (mol/mol) in the production phase. To our knowledge, this work is the first to produce isopropanol in E. coli, and the titer exceeded that from the native producers.

元の言語英語
ページ(範囲)7814-7818
ページ数5
ジャーナルApplied and Environmental Microbiology
73
発行部数24
DOI
出版物ステータス出版済み - 12 1 2007

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2-Propanol
isopropyl alcohol
Clostridium beijerinckii
alcohol
Escherichia coli
Thermoanaerobacter brockii
gene
acetoacetate decarboxylase
Clostridium acetobutylicum
Escherichia coli K12
acetyl coenzyme A
acetyltransferases
Thermoanaerobacter
Acetyl-CoA C-Acetyltransferase
alcohol dehydrogenase
Clostridium
Population Groups
genes
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

これを引用

Engineered synthetic pathway for isopropanol production in Escherichia coli. / Hanai, Taizo; Atsumi, S.; Liao, J. C.

:: Applied and Environmental Microbiology, 巻 73, 番号 24, 01.12.2007, p. 7814-7818.

研究成果: ジャーナルへの寄稿記事

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