Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light

Tamami Kusakabe, Tsuneyuki Tatsuke, Keigo Tsuruno, Yasutaka Hirokawa, Shota Atsumi, James C. Liao, Taizo Hanai

研究成果: Contribution to journalArticle査読

100 被引用数 (Scopus)

抄録

Production of alternate fuels or chemicals directly from solar energy and carbon dioxide using engineered cyanobacteria is an attractive method to reduce petroleum dependency and minimize carbon emissions. Here, we constructed a synthetic pathway composed of acetyl-CoA acetyl transferase (encoded by thl), acetoacetyl-CoA transferase (encoded by atoAD), acetoacetate decarboxylase (encoded by adc) and secondary alcohol dehydrogenase (encoded by adh) in Synechococcus elongatus strain PCC 7942 to produce isopropanol. The enzyme-coding genes, heterogeneously originating from Clostridium acetobutylicum ATCC 824 (thl and adc), Escherichia coli K-12 MG1655 (atoAD) and Clostridium beijerinckii (adh), were integrated into the S. elongatus genome. Under the optimized production conditions, the engineered cyanobacteria produced 26.5. mg/L of isopropanol after 9 days.

本文言語英語
ページ(範囲)101-108
ページ数8
ジャーナルMetabolic Engineering
20
DOI
出版ステータス出版済み - 11 2013

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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