Engineering of capillary-like structures in tissue constructs by electrochemical detachment of cells

Yuki Seto, Rina Inaba, Tomoaki Okuyama, Fumihiro Sassa, Hiroaki Suzuki, Junji Fukuda

研究成果: Contribution to journalArticle査読

57 被引用数 (Scopus)

抄録

A major challenge in the development of functional thick tissues is the formation of vascular networks for oxygen and nutrient supply throughout the engineered tissue constructs. This study describes an electrochemical approach for fabrication of capillary-like structures, precisely aligned within micrometer distances, whose internal surfaces are covered with vascular endothelial cells. In this approach, an oligopeptide containing a cell adhesion domain (RGD) in the center and cysteine residues at both ends was designed. Cysteine has a thiol group that adsorbs onto a gold surface via a gold-thiolate bond. The cells attached to the gold surface via the oligopeptide were readily and noninvasively detached by applying a negative electrical potential and cleaving the gold-thiolate bond. This approach was applicable not only for a flat surface but also for various configurations, including cylindrical structures. By applying this approach to thin gold rods aligned in a spatially controlled manner in a perfusion culture device, human umbilical vein endothelial cells (HUVECs) were transferred onto the internal surface of capillary structures in collagen gel. In the subsequent perfusion culture, the HUVECs grew into the collagen gel and formed luminal structures, thereby forming vascular networks in vitro.

本文言語英語
ページ(範囲)2209-2215
ページ数7
ジャーナルBiomaterials
31
8
DOI
出版ステータス出版済み - 3 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオエンジニアリング
  • セラミックおよび複合材料
  • 生物理学
  • 生体材料
  • 材料力学

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