Enhanced expression of brain interferon-α and serotonin transporter in immunologically induced fatigue in rats

Toshihiko Katafuchi, Tetsuya Kondo, Sachiko Take, Megumu Yoshimura

研究成果: ジャーナルへの寄稿記事

63 引用 (Scopus)

抄録

Immunologically induced fatigue was induced in rats by intraperitoneal injection of a synthetic double-stranded RNA, polyriboinosinic: polyribocytidylic acid (poly I:C). An injection of poly I:C (3 mg/kg) decreased the daily amounts of spontaneous running wheel activity to ≈60% of the preinjection level until day 8. Quantitative analysis of mRNA levels demonstrated that interferon-α (IFN-α) and p38 mitogen-activated protein kinase mRNAs increased in the medial preoptic, paraventricular and ventromedial hypothalamic nuclei and in cortex on both days 1 and 8, while interleukin-1β and an inhibitor of nuclear factor κB (IκB)-β mRNAs increased on day 1, but recovered within a week. Serotonin transporter (5-HTT) mRNA also increased on days 1 and 8 after poly I:C injection in the same brain regions where IFN-α mRNA increased. The increased 5-HTT had a functional significance, because in vivo brain microdialysis revealed that an i.p. injection of poly I:C induced a decrease in the extracellular concentration of 5-HT in the prefrontal cortex; the decrease was blocked by local perfusion with a nonselective 5-HT reuptake inhibitor, imipramine. Finally, the poly I:C-induced fatigue was attenuated by a 5-HT 1A receptor agonist but not by 5-HT2, 5-HT3 or dopamine D3 agonists. These findings, taken together, suggest that disorders in brain IFN-α and 5-HTT expression may be involved in the neuronal mechanisms of the poly I:C-induced fatigue.

元の言語英語
ページ(範囲)2817-2826
ページ数10
ジャーナルEuropean Journal of Neuroscience
22
発行部数11
DOI
出版物ステータス出版済み - 12 1 2005

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Serotonin Plasma Membrane Transport Proteins
Interferons
Fatigue
Brain
Messenger RNA
Injections
Serotonin
Serotonin 5-HT2 Receptor Agonists
Ventromedial Hypothalamic Nucleus
Receptor, Serotonin, 5-HT1A
Double-Stranded RNA
Paraventricular Hypothalamic Nucleus
Imipramine
Dopamine Agonists
Microdialysis
Brain Diseases
p38 Mitogen-Activated Protein Kinases
Prefrontal Cortex
Intraperitoneal Injections
Interleukin-1

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

これを引用

Enhanced expression of brain interferon-α and serotonin transporter in immunologically induced fatigue in rats. / Katafuchi, Toshihiko; Kondo, Tetsuya; Take, Sachiko; Yoshimura, Megumu.

:: European Journal of Neuroscience, 巻 22, 番号 11, 01.12.2005, p. 2817-2826.

研究成果: ジャーナルへの寄稿記事

Katafuchi, Toshihiko ; Kondo, Tetsuya ; Take, Sachiko ; Yoshimura, Megumu. / Enhanced expression of brain interferon-α and serotonin transporter in immunologically induced fatigue in rats. :: European Journal of Neuroscience. 2005 ; 巻 22, 番号 11. pp. 2817-2826.
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