Enhanced liver functions in mouse hepatoma cells by induced overexpression of liver-enriched transcription factors

Hideaki Yamamoto, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

Hepatoma cells, which are derived from liver carcinoma, are able to proliferate infinitely under culture conditions. However, the liver functions of hepatoma cells are generally low compared with those of hepatocytes in a liver. Here, we attempted to create genetically engineered hepatoma cells with enhanced liver functions by overexpression of liver-enriched transcription factors (LETFs), which are associated with the transcription of liver-specific genes and hepatic differentiation. For this purpose, genes for eight LETFs, hepatocyte nuclear factor (HNF)-1α, HNF-1β, HNF-3β, HNF-4α, HNF-6, CCAAT/enhancer binding protein (C/EBP)-α, C/EBP-β and C/EBP-γ, were obtained from the mouse liver. Mouse hepatoma Hepa1-6 cells were transduced with retroviral vectors, in which inducible expression cassettes for the LETF genes were introduced. Cell clones with inducible expression of high liver functions were established. Upon overexpression of the LETF genes, cell proliferation ceased and the cells exhibited an epithelial morphology, indicating hepatic maturation of hepatoma cells. This approach for genetic modification of hepatoma cells may be promising for the construction of cells for use in bioartificial liver support systems.

本文言語英語
ページ(範囲)67-73
ページ数7
ジャーナルBiochemical Engineering Journal
60
DOI
出版ステータス出版済み - 1 15 2012

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 環境工学
  • バイオエンジニアリング
  • 生体医工学

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