Enhanced sensitivity of a surface-plasmon-resonance (SPR) sensor for 2,4-D by controlled functionalization of self-assembled monolayer-based immunosensor chip

Sook Jin Kim, K. Vengatajalabathy Gobi, Hiroyuki Tanaka, Yukihiro Shoyama, Norio Miura

研究成果: ジャーナルへの寄稿記事

18 引用 (Scopus)

抄録

A highly sensitive SPR immunosensor has been fabricated by covalent binding of a protein conjugate of 2,4-D (2,4-D-BSA) on SAM of 3-mercaptopropionic acid. With indirect inhibition immunoassay, it demonstrated a low detection limit of 0.1 ppb 2,4-D. Taking advantage of the combination of the SAM technique and immobilization of 2,4-D-BSA at controlled surface coverage, a remarkable enhancement of the low detection limit by 10-fold to 10 ppt with no use of any labels was demonstrated, while keeping the response time constant at 4 min.

元の言語英語
ページ(範囲)1132-1133
ページ数2
ジャーナルChemistry Letters
35
発行部数10
DOI
出版物ステータス出版済み - 10 5 2006

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Immunosensors
2,4-Dichlorophenoxyacetic Acid
Surface plasmon resonance
Self assembled monolayers
Sensors
3-Mercaptopropionic Acid
Labels
Proteins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

これを引用

Enhanced sensitivity of a surface-plasmon-resonance (SPR) sensor for 2,4-D by controlled functionalization of self-assembled monolayer-based immunosensor chip. / Kim, Sook Jin; Gobi, K. Vengatajalabathy; Tanaka, Hiroyuki; Shoyama, Yukihiro; Miura, Norio.

:: Chemistry Letters, 巻 35, 番号 10, 05.10.2006, p. 1132-1133.

研究成果: ジャーナルへの寄稿記事

Kim, Sook Jin ; Gobi, K. Vengatajalabathy ; Tanaka, Hiroyuki ; Shoyama, Yukihiro ; Miura, Norio. / Enhanced sensitivity of a surface-plasmon-resonance (SPR) sensor for 2,4-D by controlled functionalization of self-assembled monolayer-based immunosensor chip. :: Chemistry Letters. 2006 ; 巻 35, 番号 10. pp. 1132-1133.
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abstract = "A highly sensitive SPR immunosensor has been fabricated by covalent binding of a protein conjugate of 2,4-D (2,4-D-BSA) on SAM of 3-mercaptopropionic acid. With indirect inhibition immunoassay, it demonstrated a low detection limit of 0.1 ppb 2,4-D. Taking advantage of the combination of the SAM technique and immobilization of 2,4-D-BSA at controlled surface coverage, a remarkable enhancement of the low detection limit by 10-fold to 10 ppt with no use of any labels was demonstrated, while keeping the response time constant at 4 min.",
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