Enhancement of acetone-butanol-ethanol fermentation from eucalyptus hydrolysate with optimized nutrient supplementation through statistical experimental designs

Jin Zheng, Yukihiro Tashiro, Tao Zhao, Qunhui Wang, Kenji Sakai, Kenji Sonomoto

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

Eucalyptus has been previously suggested as a potential substrate for acetone-butanol-ethanol (ABE) production without nutrient supplementation; however, incomplete sugar consumption has prevented improvement of ABE production. Cellulase loading with 35 FPU g−1 was first optimized in terms of high hydrolysis efficiency (95%). However, only 0.43 g L−1 ABE production and 3.44% glucose consumption rate were achieved. To improve ABE production from eucalyptus hydrolysate, supplementation of 6 nutrients in common tryptone-yeast extract (TY) medium were investigated by statistical approaches. Three nutrients including yeast extract, tryptone, and FeSO4·7H2O were screened as significant nutrients for ABE production. Subsequently, use of a modified TY medium (MTY medium: yeast extract 3.04 g L−1, tryptone 7.64 g L−1, FeSO4·7H2O 15.3 mg L−1), which was subsequently predicted by Plackett-Burman and Box-Behnken designs to stimulate ABE production, resulted in ca. 40-fold increase in ABE concentration (16.9 g L−1) and a glucose consumption rate of 100%. We first examined previously uninvestigated nutrition combinations using Plackett-Burman and Box-Behnken designs for high ABE production from eucalyptus hydrolysate. This study shows that statistical method would be a powerful tool for the optimization and enhancement of ABE production from eucalyptus hydrolysate.

本文言語英語
ページ(範囲)580-586
ページ数7
ジャーナルRenewable Energy
113
DOI
出版ステータス出版済み - 2017

All Science Journal Classification (ASJC) codes

  • 再生可能エネルギー、持続可能性、環境

フィンガープリント

「Enhancement of acetone-butanol-ethanol fermentation from eucalyptus hydrolysate with optimized nutrient supplementation through statistical experimental designs」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル