Enhancement of activity of sol-gel immobilized lipase in organic media by pretreatment with substrate analogues

Shin Ya Furukawa, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

研究成果: Contribution to journalArticle査読

14 被引用数 (Scopus)

抄録

By performing pretreatment with substrate analogues, an activation of lipase immobilized into organic-inorganic hybrid silicate was attempted in esterification of menthol with butyric acid in organic media. With decreasing water content of immobilized lipase preparations, the activity of immobilized lipases by (-)-menthol pretreatment was enhanced, and the highest activity was obtained at water activity, aw=0.11. However, the pretreatment of immobilized lipases with (-)-menthol could not improve the enantioselectivity. In addition, the activity of pretreated immobilized lipases increased with increasing the chain length of alcohol as a substrate analogue. Consequently, it was found that hydrophobic and bulky alcohol was an effective substrate analogue to activate immobilized lipases.

本文言語英語
ページ(範囲)65-70
ページ数6
ジャーナルJournal of Molecular Catalysis - B Enzymatic
15
1-3
DOI
出版ステータス出版済み - 9 28 2001

All Science Journal Classification (ASJC) codes

  • 触媒
  • バイオエンジニアリング
  • 生化学
  • プロセス化学およびプロセス工学

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