Enhancement of apparent thermostability of lipase from Rhizopus sp. by the treatment with a microbial transglutaminase

Noriho Kamiya; Takashi Ogawa; Teruyuki Nagamune

doi:10.1023/A:1011936618341

Enhancement of apparent thermostability of lipase from Rhizopus sp. by the treatment with a microbial transglutaminase

Noriho Kamiya, Takashi Ogawa, Teruyuki Nagamune

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

11 被引用数 (Scopus)

抄録

Crude lipase from Rhizopus sp. was moderately stable against heat treatment at 45 °C. However, after incubation for 1 h at 25 °C with Streptoverticillium transglutaminase (MTG), the half-life of crude lipase in the heat treatment was increased more than 10-fold compared to that of untreated one. The result can be ascribed by the MTGasemediated crosslinking of contaminating proteins that affect the apparent thermostability of lipase in the crude sample.

本文言語	英語
ページ（範囲）	1629-1632
ページ数	4
ジャーナル	Biotechnology letters
巻	23
号	19
DOI	https://doi.org/10.1023/A:1011936618341
出版ステータス	出版済み - 2001
外部発表	はい

!!!All Science Journal Classification (ASJC) codes

バイオテクノロジー
バイオエンジニアリング
応用微生物学とバイオテクノロジー

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10.1023/A:1011936618341

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title = "Enhancement of apparent thermostability of lipase from Rhizopus sp. by the treatment with a microbial transglutaminase",

abstract = "Crude lipase from Rhizopus sp. was moderately stable against heat treatment at 45 °C. However, after incubation for 1 h at 25 °C with Streptoverticillium transglutaminase (MTG), the half-life of crude lipase in the heat treatment was increased more than 10-fold compared to that of untreated one. The result can be ascribed by the MTGasemediated crosslinking of contaminating proteins that affect the apparent thermostability of lipase in the crude sample.",

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year = "2001",

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T1 - Enhancement of apparent thermostability of lipase from Rhizopus sp. by the treatment with a microbial transglutaminase

AU - Kamiya, Noriho

AU - Ogawa, Takashi

AU - Nagamune, Teruyuki

PY - 2001

Y1 - 2001

N2 - Crude lipase from Rhizopus sp. was moderately stable against heat treatment at 45 °C. However, after incubation for 1 h at 25 °C with Streptoverticillium transglutaminase (MTG), the half-life of crude lipase in the heat treatment was increased more than 10-fold compared to that of untreated one. The result can be ascribed by the MTGasemediated crosslinking of contaminating proteins that affect the apparent thermostability of lipase in the crude sample.

AB - Crude lipase from Rhizopus sp. was moderately stable against heat treatment at 45 °C. However, after incubation for 1 h at 25 °C with Streptoverticillium transglutaminase (MTG), the half-life of crude lipase in the heat treatment was increased more than 10-fold compared to that of untreated one. The result can be ascribed by the MTGasemediated crosslinking of contaminating proteins that affect the apparent thermostability of lipase in the crude sample.

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Enhancement of apparent thermostability of lipase from Rhizopus sp. by the treatment with a microbial transglutaminase

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!!!All Science Journal Classification (ASJC) codes

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