Enhancement of transfection efficiency using ligand-modified lipid vesicles

Jun You, Masamichi Kamihira, Shinji Iijima

研究成果: Contribution to journalArticle査読

12 被引用数 (Scopus)

抄録

Previously, we had developed a simple gene transfection technique for animal cells using cationic lipid vesicles; a commercially available synthetic cationic surfactant, dimethyldioctadecyl ammonium bromide (DDAB) was used for making lipid vesicles. In the present study, the lipid vesicles for receptor-mediated gene transfer were modified with a ligand such as insulin and galactose residues to realize enhanced transfection efficiency and/or cell-specific gene transfection. The insulin-modified lipid vesicle solution mixed with the plasmid DNA (pCMVβ) was added to COS-7, NIH3T3, Hela or HepG2 cells; the transfection efficiency was increased 3-4-fold in all the cell lines tested. Furthermore, a mixture of the galactose-modified lipid vesicles and plasmid pCMVβ was added to HepG2 or HUH-6 cells expressing asialoglycoprotein receptors, and the transfection efficiency was increased 3-4-fold in these cell lines.

本文言語英語
ページ(範囲)525-528
ページ数4
ジャーナルJournal of Fermentation and Bioengineering
85
5
DOI
出版ステータス出版済み - 1998
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

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