Enzymatic assay of phosphatidylethanolamine in serum using amine oxidase from Arthrobacter sp

Eisaku Hokazono, Hideto Tamezane, Taeko Hotta, Yuzo Kayamori, Susumu Osawa

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Background: In human serum, as for phospholipids not containing choline, phosphatidylethanolamine (PE) exists approximately 5% in a whole phospholipid. PE is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis and cell signaling. However, it could not measure PE with other phospholipids due to a lack of choline in them. Methods: Using an amine oxidase (EC 1.4.3.6), from Arthrobacter species, a simple and rapid enzymatic assay for measurements of PE in serum was established. That assay used the Hitachi 7170 analyzer to evaluate the analytical performance. Results: The average within-run CVs were 0.38-1.27% (n=20) at 69-160μmol/l. The correlation between values obtained with the present method (y) and the high-performance liquid chromatography (HPLC) method (x) was: y=0.944x+9.441 (r=0.977, Sy|x=5.82, n=34). In addition, the reference interval of healthy subjects was 115±45μmol/l. Conclusions: This new enzymatic method shows a high specificity for serum PE and can be easily applied to an automated analyzer. The present method is available as a novel marker of changes in the clinical condition of serum phospholipids.

元の言語英語
ページ(範囲)1436-1440
ページ数5
ジャーナルClinica Chimica Acta
412
発行部数15-16
DOI
出版物ステータス出版済み - 7 15 2011

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Arthrobacter
Enzyme Assays
Amines
Assays
Oxidoreductases
Phospholipids
Serum
Choline
Cell signaling
Biological membranes
High performance liquid chromatography
Healthy Volunteers
High Pressure Liquid Chromatography
phosphatidylethanolamine
Apoptosis
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

これを引用

Enzymatic assay of phosphatidylethanolamine in serum using amine oxidase from Arthrobacter sp. / Hokazono, Eisaku; Tamezane, Hideto; Hotta, Taeko; Kayamori, Yuzo; Osawa, Susumu.

:: Clinica Chimica Acta, 巻 412, 番号 15-16, 15.07.2011, p. 1436-1440.

研究成果: ジャーナルへの寄稿記事

Hokazono, Eisaku ; Tamezane, Hideto ; Hotta, Taeko ; Kayamori, Yuzo ; Osawa, Susumu. / Enzymatic assay of phosphatidylethanolamine in serum using amine oxidase from Arthrobacter sp. :: Clinica Chimica Acta. 2011 ; 巻 412, 番号 15-16. pp. 1436-1440.
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