Enzymatic characterization of two epsilon-class glutathione S-transferases of Spodoptera litura

Aiko Hirowatari, Zhiwei Chen, Kazuei Mita, Kohji Yamamoto

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

Two cDNAs encoding glutathione S-transferase (GST) of the tobacco cutworm, Spodoptera litura, were cloned by reverse transcriptase-polymerase chain reaction. The deduced amino acid sequences of the resulting clones revealed 32–51% identities to the epsilon-class GSTs from other organisms. The recombinant proteins were functionally overexpressed in Escherichia coli cells in soluble form and were purified to homogeneity. The enzymes were capable of catalyzing the bioconjugation of glutathione with 1-chloro-2,4-dinitrobenzene, 1,2-epoxy-3-(4-nitrophenoxy)-propane, and ethacrynic acid. A competition assay revealed that the GST activity was inhibited by insecticides, suggesting that it could be conducive to insecticide tolerance in the tobacco cutworm.

本文言語英語
論文番号e21443
ジャーナルArchives of insect biochemistry and physiology
97
3
DOI
出版ステータス出版済み - 3 2018

All Science Journal Classification (ASJC) codes

  • 生理学
  • 生化学
  • 昆虫科学

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