Enzymatic characterization of two epsilon-class glutathione S-transferases of Spodoptera litura

Aiko Hirowatari, Zhiwei Chen, Kazuei Mita, Kohji Yamamoto

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

Two cDNAs encoding glutathione S-transferase (GST) of the tobacco cutworm, Spodoptera litura, were cloned by reverse transcriptase-polymerase chain reaction. The deduced amino acid sequences of the resulting clones revealed 32–51% identities to the epsilon-class GSTs from other organisms. The recombinant proteins were functionally overexpressed in Escherichia coli cells in soluble form and were purified to homogeneity. The enzymes were capable of catalyzing the bioconjugation of glutathione with 1-chloro-2,4-dinitrobenzene, 1,2-epoxy-3-(4-nitrophenoxy)-propane, and ethacrynic acid. A competition assay revealed that the GST activity was inhibited by insecticides, suggesting that it could be conducive to insecticide tolerance in the tobacco cutworm.

元の言語英語
記事番号e21443
ジャーナルArchives of insect biochemistry and physiology
97
発行部数3
DOI
出版物ステータス出版済み - 3 1 2018

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Spodoptera
Spodoptera litura
Tobacco
Insecticides
Glutathione Transferase
glutathione transferase
Ethacrynic Acid
Dinitrochlorobenzene
Propane
Polymerase chain reaction
RNA-Directed DNA Polymerase
insecticides
ethacrynic acid
Reverse Transcriptase Polymerase Chain Reaction
Recombinant Proteins
Escherichia coli
Glutathione
Amino Acid Sequence
Assays
propane

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Insect Science

これを引用

Enzymatic characterization of two epsilon-class glutathione S-transferases of Spodoptera litura. / Hirowatari, Aiko; Chen, Zhiwei; Mita, Kazuei; Yamamoto, Kohji.

:: Archives of insect biochemistry and physiology, 巻 97, 番号 3, e21443, 01.03.2018.

研究成果: ジャーナルへの寄稿記事

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