Enzymatic degradation of p-chlorophenol in a two-phase flow microchannel system

Tatsuo Maruyama, Jun Ichi Uchida, Tomohiro Ohkawa, Toru Futami, Koji Katayama, Kei Ichiro Nishizawa, Ken Ichiro Sotowa, Fukiko Kubota, Noriho Kamiya, Masahiro Goto

研究成果: ジャーナルへの寄稿学術誌査読

94 被引用数 (Scopus)


Enzymatic degradation of p-chlorophenol was carried out in a two-phase flow in a microchannel (100 μm width, 25 μm depth) fabricated on a glass plate (70 mm × 38 mm). This is the first report on the enzymatic reaction in a two-phase flow on a microfluidic device. The surface of the microchannel was partially modified with octadecylsilane groups to be hydrophobic, thus allowing clear phase separation at the end-junction of the microchannel. The enzyme (laccase), which is surface active, was solubilized in a succinic aqueous buffer and the substrate (p-chlorophenol) was in isooctane. The degradation of p-chlorophenol occurred mainly at the aqueous-organic interface in the microchannel. We investigated the effects of flow velocity and microchannel shape on the enzymatic degradation of p-chlorophenol. Assuming that diffusion of the substrate (p-chlorophenol) is the rate-limiting step in the enzymatic degradation of p-chlorophenol in the microchannel, we proposed a simple theoretical model for the degradation in the microchannel. The calculated degradation values agreed well with the experimental data.

ジャーナルLab on a Chip
出版ステータス出版済み - 11月 2003

!!!All Science Journal Classification (ASJC) codes

  • バイオエンジニアリング
  • 生化学
  • 化学 (全般)
  • 生体医工学


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