Enzymatic Esterification by Surfactant‐Coated Lipase in Organic Media

Masahiro Goto, Noriho Kamiya, Masaki Miyata, Fumiyuki Nakashio

研究成果: Contribution to journalArticle査読

95 被引用数 (Scopus)

抄録

Surfactant‐coated lipases have been prepared with a synthesized surfactant. Preparation conditions to obtain a suitable surfactant‐coated lipase were investigated. The enzymatic activity of the lipase in an organic solvent significantly increased with the coating of the surfactant. The esterification rate from the surfactant‐coated lipase was much higher than that from the powder lipase. An aliphatic solvent showed higher activity than did alcohol, aromatic, and chloric solvents. Among them, isooctane gave the highest activity. The reactivity of the surfactant‐coated lipase depends on the pH of the aqueous solution in the preparation and on the buffer solution. Surfactant‐coated lipase prepared in the middle pH range using phosphate buffer showed high enzymatic activity. The surfactant‐coated lipase was thermostable at high temperature compared to the native lipase. A kinetic study enabled a ping‐pong bi‐bi reaction mechanism with alcohol inhibition to be suggested. From the kinetic analysis, it was found that an alcohol substrate inhibits enzymatic esterification by lipase. The reaction rate of the coated lipase was about 100 times that of the powder lipase.

本文言語英語
ページ(範囲)263-268
ページ数6
ジャーナルBiotechnology Progress
10
3
DOI
出版ステータス出版済み - 1 1 1994

All Science Journal Classification (ASJC) codes

  • Biotechnology

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