Enzymatic hydrolysis of soluble starch in a polyethylene glycol-dextran aqueous two-phase system

Kazuhiko Hayashida, Koji Kunimoto, Fumihide Shiraishi, Koei Kawakami, Yasuhiko Arai

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

Hydrolysis of soluble starch by glucoamylase and β-amylase was investigated as a model reaction in an aqueous two-phase system consisting of polyethylene glycol (PEG) and dextran (DEX). Changes in glucose concentration observed in the batch reaction experiments with glucoamylase were almost identical for the aqueous two-phase and pure water systems, showing that the enzymic reactions investigated were not influenced by the presence of PEG and DEX. The partition of β-amylase into the DEX phase was insufficient compared to that of glucoamylase. Hence, the former enzyme was crosslinked with glutaraldehyde to increase its apparent molecular weight and, as a consequence, the partition coefficient, defined as the concentration ratio of the component partitioned into the PEG phase to that into the DEX phase, was decreased to 17% of that of the original enzyme. In the operation in which the enzyme and substrate are partitioned selectively into the DEX phase and allowed to react there while the product, thus transferring to the PEG phase, is recovered, the aqueous two-phase system with a smaller partition coefficient provided longer operational stability.

元の言語英語
ページ(範囲)240-243
ページ数4
ジャーナルJournal of Fermentation and Bioengineering
69
発行部数4
DOI
出版物ステータス出版済み - 1 1 1990

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Enzymatic hydrolysis
Dextran
Dextrans
Starch
Polyethylene glycols
Hydrolysis
Glucan 1,4-alpha-Glucosidase
Amylases
Enzymes
Glutaral
Glucose
Molecular Weight
Molecular weight
Water
Substrates
Experiments

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

これを引用

Enzymatic hydrolysis of soluble starch in a polyethylene glycol-dextran aqueous two-phase system. / Hayashida, Kazuhiko; Kunimoto, Koji; Shiraishi, Fumihide; Kawakami, Koei; Arai, Yasuhiko.

:: Journal of Fermentation and Bioengineering, 巻 69, 番号 4, 01.01.1990, p. 240-243.

研究成果: ジャーナルへの寄稿記事

Hayashida, Kazuhiko ; Kunimoto, Koji ; Shiraishi, Fumihide ; Kawakami, Koei ; Arai, Yasuhiko. / Enzymatic hydrolysis of soluble starch in a polyethylene glycol-dextran aqueous two-phase system. :: Journal of Fermentation and Bioengineering. 1990 ; 巻 69, 番号 4. pp. 240-243.
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abstract = "Hydrolysis of soluble starch by glucoamylase and β-amylase was investigated as a model reaction in an aqueous two-phase system consisting of polyethylene glycol (PEG) and dextran (DEX). Changes in glucose concentration observed in the batch reaction experiments with glucoamylase were almost identical for the aqueous two-phase and pure water systems, showing that the enzymic reactions investigated were not influenced by the presence of PEG and DEX. The partition of β-amylase into the DEX phase was insufficient compared to that of glucoamylase. Hence, the former enzyme was crosslinked with glutaraldehyde to increase its apparent molecular weight and, as a consequence, the partition coefficient, defined as the concentration ratio of the component partitioned into the PEG phase to that into the DEX phase, was decreased to 17{\%} of that of the original enzyme. In the operation in which the enzyme and substrate are partitioned selectively into the DEX phase and allowed to react there while the product, thus transferring to the PEG phase, is recovered, the aqueous two-phase system with a smaller partition coefficient provided longer operational stability.",
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AU - Arai, Yasuhiko

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N2 - Hydrolysis of soluble starch by glucoamylase and β-amylase was investigated as a model reaction in an aqueous two-phase system consisting of polyethylene glycol (PEG) and dextran (DEX). Changes in glucose concentration observed in the batch reaction experiments with glucoamylase were almost identical for the aqueous two-phase and pure water systems, showing that the enzymic reactions investigated were not influenced by the presence of PEG and DEX. The partition of β-amylase into the DEX phase was insufficient compared to that of glucoamylase. Hence, the former enzyme was crosslinked with glutaraldehyde to increase its apparent molecular weight and, as a consequence, the partition coefficient, defined as the concentration ratio of the component partitioned into the PEG phase to that into the DEX phase, was decreased to 17% of that of the original enzyme. In the operation in which the enzyme and substrate are partitioned selectively into the DEX phase and allowed to react there while the product, thus transferring to the PEG phase, is recovered, the aqueous two-phase system with a smaller partition coefficient provided longer operational stability.

AB - Hydrolysis of soluble starch by glucoamylase and β-amylase was investigated as a model reaction in an aqueous two-phase system consisting of polyethylene glycol (PEG) and dextran (DEX). Changes in glucose concentration observed in the batch reaction experiments with glucoamylase were almost identical for the aqueous two-phase and pure water systems, showing that the enzymic reactions investigated were not influenced by the presence of PEG and DEX. The partition of β-amylase into the DEX phase was insufficient compared to that of glucoamylase. Hence, the former enzyme was crosslinked with glutaraldehyde to increase its apparent molecular weight and, as a consequence, the partition coefficient, defined as the concentration ratio of the component partitioned into the PEG phase to that into the DEX phase, was decreased to 17% of that of the original enzyme. In the operation in which the enzyme and substrate are partitioned selectively into the DEX phase and allowed to react there while the product, thus transferring to the PEG phase, is recovered, the aqueous two-phase system with a smaller partition coefficient provided longer operational stability.

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