Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae

Kaoru Takegawa, S. Yamaguchi, A. Kondo, I. Kato, S. Iwahara

研究成果: Contribution to journalArticle査読

45 被引用数 (Scopus)

抄録

The transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. When (Man)6(GlcNAc)2Asn was used as a substrate for the transglycosylation, (Man)6GlcNAc-Glc, (Man)6GlcNAc-Man, (Man)6GlcNAc-chitobiose, and (Man)6GlcNAc-gentiobiose were synthesized. Their structures were identified by HPLC, ion spray mass spectrometry, and digestion with glycosidases Endo-β-N-acetylglucosaminidases hydrolyzed the pyridylamino derivatives of these oligosaccharides.

本文言語英語
ページ(範囲)829-835
ページ数7
ジャーナルBiochemistry International
25
5
出版ステータス出版済み - 1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biochemistry

フィンガープリント 「Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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