Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

Takao Yasui, Koki Motoyama, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

研究成果: Contribution to journalArticle

抄録

The technical development of long-term fluorescent observation of single DNA molecules is central to fields ranging from molecular biological detection to understanding the physical properties of them under a microscope. Here, we address this challenge using protocatechuic acid and protocatechuate-3,4- dioxygenase (PADase) and demonstrate fluorescent lifetimes of dyed single DNA molecules of 150-180 s, three times longer than those without any treatments.

本文言語英語
ページ(範囲)3237-3240
ページ数4
ジャーナルRSC Advances
3
10
DOI
出版ステータス出版済み - 3 14 2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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