Enzyme-linked immunosorbent assay for the determination of total ginsenosides in ginseng

Osamu Morinaga, Hiroyuki Tanaka, Yukihiro Shoyama

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

Hybridoma secreting a monoclonal antibody (MAb) against ginsenosides was produced by fusing splenocytes from a mouse immunized against a ginsenoside Re-bovine serum albumin (G-Re-BSA) conjugate with myeloma cell line SP2/0-Ag14. A MAb-4G10 had a wide cross-reaction with 20(5)-protopanaxadiol and 20(S)-protopanaxatriol type ginsenosides. An enzyme-linked immunosorbent assay (ELISA) that had an effective measuring range of 20-400 ng/mL for total ginsenosides when G-Re was used as a standard. Total ginsenosides concentration in ginseng were determined by ELISA and high-performance liquid chromatography (HPLC) in a good agreement together, and the least-squares fit had a coefficient of determination (γ2) of 0.995.

元の言語英語
ページ(範囲)287-296
ページ数10
ジャーナルAnalytical Letters
39
発行部数2
DOI
出版物ステータス出版済み - 1 1 2006

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Ginsenosides
Immunosorbents
Panax
Monoclonal antibodies
Assays
Enzymes
Enzyme-Linked Immunosorbent Assay
High performance liquid chromatography
Monoclonal Antibodies
Cells
Cross Reactions
Hybridomas
Bovine Serum Albumin
Least-Squares Analysis
High Pressure Liquid Chromatography
Cell Line

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

これを引用

Enzyme-linked immunosorbent assay for the determination of total ginsenosides in ginseng. / Morinaga, Osamu; Tanaka, Hiroyuki; Shoyama, Yukihiro.

:: Analytical Letters, 巻 39, 番号 2, 01.01.2006, p. 287-296.

研究成果: ジャーナルへの寄稿記事

Morinaga, Osamu ; Tanaka, Hiroyuki ; Shoyama, Yukihiro. / Enzyme-linked immunosorbent assay for the determination of total ginsenosides in ginseng. :: Analytical Letters. 2006 ; 巻 39, 番号 2. pp. 287-296.
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