Enzyme-linked immunosorbent assay for total isoflavonoids in pueraria candollei using anti-puerarin and anti-daidzin polyclonal antibodies

Benyakan Pongkitwitoon, Seiichi Sakamoto, Hiroyuki Tanaka, Ryota Tsuchihashi, Junei Kinjo, Satoshi Morimoto, Waraporn Putalun

研究成果: Contribution to journalArticle査読

21 被引用数 (Scopus)

抄録

Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin, daidzin, genistin, daidzein, and genistein as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these compounds, a single enzyme-linked immunosorbent assay (ELISA) for total isoflavonoids was developed using anti-puerarin and anti-daidzin polyclonal antibodies (PAbs). The range for calibration of isoflavonoids by ELISA was 0.056.25g/mL. Total isoflavonoid concentrations in P. candollei samples determined by the newly developed assay system showed good agreement with those analyzed by HPLC. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of total isoflavonoids in P. candollei.

本文言語英語
ページ(範囲)831-836
ページ数6
ジャーナルPlanta medica
76
8
DOI
出版ステータス出版済み - 6 4 2010

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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