Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies

Osamu Morinaga, Takuhiro Uto, Seiichi Sakamoto, Hiroyuki Tanaka, Yukihiro Shoyama

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Total sennosides concentration is a very important factor when rhubarb and senna will be used as crude drugs. However, one-step analytical technique for total sennosides has not been reported except HPLC. An enzyme-linked immunosorbent assay (ELISA) for total sennosides concentration by using the combination of anti-sennoside A (SA) and anti-sennoside B (SB) monoclonal antibodies (MAbs) in a single assay has been investigated. Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively.

元の言語英語
ページ(範囲)28-31
ページ数4
ジャーナルFitoterapia
80
発行部数1
DOI
出版物ステータス出版済み - 1 1 2009

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Senna Extract
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Rheum
High Pressure Liquid Chromatography
sennoside A&B
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

これを引用

Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies. / Morinaga, Osamu; Uto, Takuhiro; Sakamoto, Seiichi; Tanaka, Hiroyuki; Shoyama, Yukihiro.

:: Fitoterapia, 巻 80, 番号 1, 01.01.2009, p. 28-31.

研究成果: ジャーナルへの寄稿記事

Morinaga, Osamu ; Uto, Takuhiro ; Sakamoto, Seiichi ; Tanaka, Hiroyuki ; Shoyama, Yukihiro. / Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies. :: Fitoterapia. 2009 ; 巻 80, 番号 1. pp. 28-31.
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