TY - JOUR
T1 - Enzyme-linked immunosorbent assay using fragment antigen-binding (Fab) antibody for determination of deoxymiroestrol, a potent phytoestrogen from Pueraria candollei
AU - Sae-Foo, Worapol
AU - Krittanai, Supaluk
AU - Juengsanguanpornsuk, Wipawee
AU - Yusakul, Gorawit
AU - Kitisripanya, Tharita
AU - Sakamoto, Seiichi
AU - Putalun, Waraporn
N1 - Funding Information:
This work was supported by the Graduate School of Khon Kaen University Faculty of Pharmaceutical Sciences, Khon Kaen University (Grant No: 631H115-C), and the International Research Network for Functional Food Discovery & Development (FFDD) of Thailand Science Research and Innovation (Grant No: IRN61W0005); Thailand Research Fund. This work was supported by the Graduate School of Khon Kaen University Faculty of Pharmaceutical Sciences, Khon Kaen University (Grant No: 631H115-C), and the International Research Network for Functional Food Discovery & Development (FFDD) of Thailand Science Research and Innovation (Grant No: IRN61W0005).
Funding Information:
This work was supported by the Graduate School of Khon Kaen University Faculty of Pharmaceutical Sciences, Khon Kaen University (Grant No: 631H115-C), and the International Research Network for Functional Food Discovery & Development (FFDD) of Thailand Science Research and Innovation (Grant No: IRN61W0005).
Publisher Copyright:
© 2021 The Author(s). Published with license by Taylor and Francis Group, LLC.
PY - 2021
Y1 - 2021
N2 - Deoxymiroestrol is the most potent phytoestrogen in the chromene group found in Pueraria candollei. The estrogenic activity of P. candollei, functions as hormone replacement therapy in postmenopausal women. This study established an enzyme-linked immunosorbent assay (ELISA) for quantifying deoxymiroestrol using fragment antigen-binding (Fab) antibody to improve analytical performance. Our ELISA specifically detected deoxymiroestrol in the range of 31.25–1,000 ng/mL. Intra- and inter-assay precision was 1.48%–7.11% and 0.58%–9.31%, respectively, the recovery of authentic deoxymiroestrol spiked into samples was 99.77%–101.61%, and the limit of detection was 30.80 ng/mL. The findings of P. candollei root bark determined using our developed ELISA with Fab antibody and an ELISA with anti-deoxymiroestrol polyclonal antibody were consistent (R2 = 0.9807). The developed ELISA can be applied to the quality control of deoxymiroestrol derived from P. candollei.
AB - Deoxymiroestrol is the most potent phytoestrogen in the chromene group found in Pueraria candollei. The estrogenic activity of P. candollei, functions as hormone replacement therapy in postmenopausal women. This study established an enzyme-linked immunosorbent assay (ELISA) for quantifying deoxymiroestrol using fragment antigen-binding (Fab) antibody to improve analytical performance. Our ELISA specifically detected deoxymiroestrol in the range of 31.25–1,000 ng/mL. Intra- and inter-assay precision was 1.48%–7.11% and 0.58%–9.31%, respectively, the recovery of authentic deoxymiroestrol spiked into samples was 99.77%–101.61%, and the limit of detection was 30.80 ng/mL. The findings of P. candollei root bark determined using our developed ELISA with Fab antibody and an ELISA with anti-deoxymiroestrol polyclonal antibody were consistent (R2 = 0.9807). The developed ELISA can be applied to the quality control of deoxymiroestrol derived from P. candollei.
UR - http://www.scopus.com/inward/record.url?scp=85109139597&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85109139597&partnerID=8YFLogxK
U2 - 10.1080/09540105.2021.1946486
DO - 10.1080/09540105.2021.1946486
M3 - Article
AN - SCOPUS:85109139597
VL - 32
SP - 336
EP - 348
JO - Food and Agricultural Immunology
JF - Food and Agricultural Immunology
SN - 0954-0105
IS - 1
ER -